A nuclear compatibility gene, Ncc-tmp, of Triticum timopheevi for the cytoplasm of Aegilops squarrosa

被引：12

作者：

Asakura, N

Nakamura, C

Ohtsuka, I

机构：

[1] KOBE UNIV, GRAD SCH SCI & TECHNOL, NADA KU, KOBE, HYOGO 657, JAPAN

[2] KOBE UNIV, FAC AGR,DEPT BIOL & ENVIRONM SCI,LAB PLANT GENET, NADA KU, KOBE, HYOGO 657, JAPAN

[3] KANAGAWA UNIV, FAC ENGN, BIOL LAB, KANAGAWA KU, YOKOHAMA, KANAGAWA 221, JAPAN

来源：

GENES & GENETIC SYSTEMS | 1997年 / 72卷 / 02期

关键词：

D O I：

10.1266/ggs.72.71

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A nuclear genome of timopheevi wheat is compatible with the cytoplasm of Aegilops squarrosa, while that of durum wheat is incompatible, thus no euploid hybrids with this cytoplasm could be obtained. A euploid nucleus-cytoplasm (NC) hybrid of Triticum durum cv. Langdon was produced by introgressing a NC compatibility gene (Ncc-tmp) of T. timopheevi. Southern blot analysis, using one RAPD marker (S13(830)) that is tightly linked to Ncc-tmp and DNAs from a series of D genome chromosome substitution lines of Langdon, demonstrated that Ncc-tmp locates on chromosome 1A. A similar function of Ncc-tmp and Ncc-sqr of Ae. squarrosa for its own cytoplasm suggested that they are homoeoallelic. Homologous sequences of two other linked RAPD markers (R03(820) and R19(600)) were detected in all accessions of T. urartu but not in T. boeoticum. The result showed the conservation of sequences adjacent to Ncc-tmp in T. urartu, an A genome donor for emmer and timopheevi wheat. An analysis of gamma-gliadin showed that the NC hybrid possesses one allele that might have been introgressed from chromosome 1G of T. timopheevi. Together with this result, 3:1 segregation of plump and shriveled seeds in BC1 of the cross between (sqr)-T. timopheevi and T. turgidum suggested the presence of an additional and functional homoeoallelic Ncc-tmp in T. timopheevi.

引用

页码：71 / 78

页数：8

共 44 条

[21] TRANSFER OF A SPECIES CYTOPLASM SPECIFIC (SCS) GENE FROM TRITICUM-TIMOPHEEVI TO TRITICUM-TURGIDUM [J].