The modification of quantum dot probes used for the targeted imaging of his-tagged fusion proteins

被引：53

作者：

Bae, Pan K. ^{[1
]}

Kim, Kyung N. ^{[1
]}

Lee, Seung J. ^{[1
]}

Chang, Hyun J. ^{[1
]}

Lee, Chong K. ^{[2
]}

Park, Joung K. ^{[1
]}

机构：

[1] Korea Res Inst Chem Technol, Adv Mat Div, Fus Biotechnol Res Ctr, Taejon 305600, South Korea

[2] Korea Res Inst Chem Technol, Drug Discovery Div, Ctr Antiinfect Agents Res, Taejon 305600, South Korea

来源：

BIOMATERIALS | 2009年 / 30卷 / 05期

关键词：

CdTe; Quantum dot; Nickel; Bio-imaging; GREEN-FLUORESCENT PROTEIN; CDSE NANOCRYSTALS; THYMIDINE KINASE; SEMICONDUCTORS; LOCALIZATION; STABILITY; PEPTIDES; CHELATE; CELLS; CDTE;

D O I：

10.1016/j.biomaterials.2008.10.049

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In molecular biology and protein detection the immobilized metal ion clusters using a NTA-chelator is a powerful technique in identification and isolation of histidine-tagged fusion proteins. The Oligohistidine tag should serve as a high affinity binding sequence for the purification of any fusion protein via metal chelating adsorbents. We described the preparation and characterization of bioinorganic conjugates made with highly luminescent semiconductor CdTe-CdS core-shell quantum dots (QDs) for biological labeling. A biocompatible surface-functionalized nanoparticle was designed to sense histidine-tagged fusion proteins. This study demonstrates the synthesis of Ni-NTA conjugated QD nanoparticles and the successful application of these nanoparticles to the detection of histicline-tagged fusion proteins. It is believed that this approach will provide a more convenient methodology for the intracellular localization of histidine-tagged protein, as compared with current methods. These Ni-NTA-QD clusters were shown to target the 6x histidine region of tagged proteins specially. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：836 / 842

页数：7

共 35 条

[31] Nanocrystalline semiconductors: Synthesis, properties, and perspectives [J].

Trindade, T ;

O'Brien, P ;

Pickett, NL .

CHEMISTRY OF MATERIALS, 2001, 13 (11) :3843-3858

[32] The green fluorescent protein [J].

Tsien, RY .

ANNUAL REVIEW OF BIOCHEMISTRY, 1998, 67 :509-544

[33] Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots [J].

Wu, XY ;

Liu, HJ ;

Liu, JQ ;

Haley, KN ;

Treadway, JA ;

Larson, JP ;

Ge, NF ;

Peale, F ;

Bruchez, MP .

NATURE BIOTECHNOLOGY, 2003, 21 (01) :41-46

[34] Formation and stability of size-, shape-, and structure-controlled CdTe nanocrystals: Ligand effects on monomers and nanocrystals [J].

Yu, WW ;

Wang, YA ;

Peng, XG .

CHEMISTRY OF MATERIALS, 2003, 15 (22) :4300-4308

[35] Experimental determination of the extinction coefficient of CdTe, CdSe, and CdS nanocrystals [J].

Yu, WW ;

Qu, LH ;

Guo, WZ ;

Peng, XG .

CHEMISTRY OF MATERIALS, 2003, 15 (14) :2854-2860

← 1 2 3 4 →