Identification and characterization of receptor for cytokine-induced neutrophil chemoattractant-3 on rat neutrophils

被引：14

作者：

Murakami, K ^{[1
]}

Shibata, F ^{[1
]}

AlMokdad, M ^{[1
]}

Nakagawa, H ^{[1
]}

Ueno, A ^{[1
]}

Kondo, T ^{[1
]}

机构：

[1] TOYAMA MED & PHARMACEUT UNIV,FAC PHARMACEUT SCI,SUGITANI,TOYAMA 93001,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 232卷 / 02期

关键词：

D O I：

10.1006/bbrc.1997.6136

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytokine-induced neutrophil chemoattractants (CINCs), members of alpha-chemokines, are considered to be major neutrophil chemotactic factors in rats. Recombinant CINC-3/rat macrophage inflammatory protein-2 (MIP-2) having an additional tyrosine residue at the carboxyl terminus (CINC-3-Tyr) was constructed, purified, radiolabeled with I-125, and used for binding studies. The specific binding of I-125-labeled CINC-3-Tyr (I-125-CINC-3-Tyr) to rat neutrophils reached a plateau after approximately 60 min at 4 degrees C. This binding of I-125-CINC-3-Tyr could be reversed by adding an excess amount of unlabeled CINC-3-Tyr. Scatchard analysis revealed approximately 12,000 binding sites per cell on rat neutrophils with an apparent Kd value of 120 pM. Chemical cross-linking experiments suggested that the rat neutrophil CINC-3 receptor is a mass of approximately 69 kDa. Taken together, our results strongly suggest that rat neutrophils express a high affinity receptor for CINC-3. (C) 1997 Academic Press.

引用

页码：562 / 567

页数：6

共 28 条

[21] LIPOPOLYSACCHARIDE-STIMULATED HUMAN MONOCYTES SECRETE, APART FROM NEUTROPHIL-ACTIVATING PEPTIDE-1 INTERLEUKIN-8, A 2ND NEUTROPHIL-ACTIVATING PROTEIN - NH2-TERMINAL AMINO-ACID-SEQUENCE IDENTITY WITH MELANOMA GROWTH STIMULATORY ACTIVITY [J].

SCHRODER, JM ;

PERSOON, NLM ;

CHRISTOPHERS, E .

JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 171 (04) :1091-1100

[22] HIGH-AFFINITY AND LOW-AFFINITY BINDING OF GRO-ALPHA AND NEUTROPHIL-ACTIVATING PEPTIDE-2 TO INTERLEUKIN-8 RECEPTORS ON HUMAN NEUTROPHILS [J].

SCHUMACHER, C ;

CLARKLEWIS, I ;

BAGGIOLINI, M ;

MOSER, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (21) :10542-10546

[23] PREVENTION OF LUNG REPERFUSION INJURY IN RABBITS BY A MONOCLONAL-ANTIBODY AGAINST INTERLEUKIN-8 [J].

SEKIDO, N ;

MUKAIDA, N ;

HARADA, A ;

NAKANISHI, I ;

WATANABE, Y ;

MATSUSHIMA, K .

NATURE, 1993, 365 (6447) :654-657

[24] RECOMBINANT PRODUCTION AND BIOLOGICAL PROPERTIES OF RAT CYTOKINE-INDUCED NEUTROPHIL CHEMOATTRACTANTS, GRO/CINC-2-ALPHA, CINC-2-BETA AND CINC-3 [J].

SHIBATA, F ;

KONISHI, K ;

KATO, H ;

KOMORITA, N ;

ALMOKDAD, M ;

FUJIOKA, M ;

NAKAGAWA, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1995, 231 (02) :306-311

[25]

WATANABE K, 1989, J BIOL CHEM, V264, P19559

[26] PURIFICATION AND CHARACTERIZATION OF CYTOKINE-INDUCED NEUTROPHIL CHEMOATTRACTANT PRODUCED BY EPITHELIOID CELL-LINE OF NORMAL RAT-KIDNEY (NRK-52E CELL) [J].

WATANABE, K ;

KINOSHITA, S ;

NAKAGAWA, H .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 161 (03) :1093-1099

[27]

WATANABE K, 1989, J PHARMACOL METHOD, V22, P13

[28] RAT CINC, A MEMBER OF THE INTERLEUKIN-8 FAMILY, IS A NEUTROPHIL-SPECIFIC CHEMOATTRACTANT INVIVO [J].

WATANABE, K ;

KOIZUMI, F ;

KURASHIGE, Y ;

TSURUFUJI, S ;

NAKAGAWA, H .

EXPERIMENTAL AND MOLECULAR PATHOLOGY, 1991, 55 (01) :30-37

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