Electrochemical characteristics of heme proteins in hydroxyethylcellulose film

被引:44
作者
Liu, XJ
Chen, T
Liu, LF
Li, GX [1 ]
机构
[1] Nanjing Univ, Dept Biochem, Nanjing 210093, Peoples R China
[2] Nanjing Univ, Natl Key Lab Pharmaceut Biotechnol, Nanjing 210093, Peoples R China
基金
中国国家自然科学基金;
关键词
Heme proteins; hydroxyethylcellulose; direct electrochemistry; biosensor;
D O I
10.1016/j.snb.2005.02.029
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A stable film made from hydroxyethylcellulose (HEC) on pyrolytic graphite (PG) electrode was employed for incorporating hemoglobin (Hb), myoglobin (Mb) and horseradish peroxidase (HRP), and the electrochemical characteristics of the proteins were studied correspondingly. Experimental results revealed that HEC film could greatly accelerate electron transfer between the proteins and electrode, and the proteins showed a thin layer electrochemical behavior in the film. Moreover, all the proteins in the film exhibited good catalytic activity towards the reduction of hydrogen peroxide (H2O2) in the low H2O2 concentration range. I it the high concentration range, H2O2 would exhibit toxicity effect on the proteins. The electrochemical properties and electrocatalytic abilities of the three heme proteins in HEC film have been compared, and the optimal conditions for H2O2 biosensor fabrication have been obtained. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:106 / 111
页数:6
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