Development of an immunosensor for human ferritin, a nonspecific tumor marker, based on a quartz crystal microbalance

被引:37
作者
Chou, SF
Hsu, WL
Hwang, JM
Chen, CY
机构
[1] Natl Taiwan Univ, Dept Agr Chem, Taipei 10764, Taiwan
[2] Chia Nan Univ Pharm & Sci, Dept Food Hlth, Tainan, Taiwan
[3] Tri Serv Gen Hosp, Natl Def Med Ctr, Dept Radiat Oncol, Taipei, Taiwan
关键词
human ferritin; immunosensor; quartz crystal microbalance (QCM); self-assembled monolayer (SAM); monoclonal antibodies (MoAbs); polyclonal antibodies (PoAbs);
D O I
10.1016/S0003-2670(01)01466-0
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A new human ferritin immunosensor was developed using anti-human ferritin antibodies (Abs) immobilized on the gold disc of a quartz crystal microbalance (QCM). Two kinds of self-assembled monolayers (SAMs) prepared by cystamine-glutaraldehyde and cystamine method were applied to immobilize anti-ferritin monoclonal antibodies (MoAbs) and polyclonal antibodies (PoAbs) on the quartz, respectively. The reusabilities of quartz crystal adopting the SAMs were found to be better than those of the other immobilization methods used. The 10 cycles of measurements could be performed on the gold surface of the same crystal regenerated with a solution of glycine.HC1. This sensor system could be continuously performed for 15 days, the relative frequency shifts (the frequency shifts measured are relative to the response at the first day) were all found to be above 95%. A linear relationship existed between the frequency shifts (Hz) and the log values of human ferritin concentrations in the range from 0.1 to 100 ng/ml in buffer and mouse serum. This human ferritin immumosensor had some advantages: high sensitivity, high specificity, low sample requirement, high reusability, no label and no pretreatment etc. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:181 / 189
页数:9
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