TT virus sequence heterogeneity in vivo:: evidence for co-infection with multiple genetic types

被引:77
作者
Ball, JK [1 ]
Curran, R
Berridge, S
Grabowska, AM
Jameson, CL
Thomson, BJ
Irving, WL
Sharp, PM
机构
[1] Univ Nottingham, Queens Med Ctr, Div Microbiol & Infect Dis, Nottingham NG7 2UH, England
[2] Univ Nottingham, Inst Genet, Nottingham NG7 2UH, England
关键词
D O I
10.1099/0022-1317-80-7-1759
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
TT virus (TTV) is a newly described DNA virus of humans that exhibits an unusually high degree of genetic heterogeneity. We have performed extensive analysis of the TTV populations present in samples, taken over a period of 2 to 6 years, from three individuals with persistent TTV infection, TTV DNA titres estimated for sequential samples were found to be quite stable over the entire study period in two patients, but fluctuated considerably in the third. DNA sequence analysis revealed different genetic diversity among TTV populations from samples from the three patients. In one case, absolute sequence homogeneity was observed among samples over a 3 year period. In a second, a limited amount of heterogeneity was found, including one sequence exhibiting G --> A hypermutation, TTV DNA sequences from the third patient exhibited quite remarkable genetic heterogeneity: evidence was found of seven distinct infecting viruses, representing four of the six TTV genotypes that have been described. In addition, minor variants of three of these seven sequences were observed, The heterogeneity of the viral population in this individual declined steadily over a 6 year period. This patient infected with a genetically diverse TTV population had the highest viral DNA titre.
引用
收藏
页码:1759 / 1768
页数:10
相关论文
共 41 条
  • [1] JC virus regulatory region rearrangements and genotypes in progressive multifocal leukoencephalopathy: Two independent aspects of virus variation
    Agostini, HT
    Ryschkewitsch, CF
    Singer, EJ
    Stoner, GL
    [J]. JOURNAL OF GENERAL VIROLOGY, 1997, 78 : 659 - 664
  • [2] HETEROGENEITY WITHIN THE EPSTEIN-BARR-VIRUS NUCLEAR ANTIGEN-2 GENE IN DIFFERENT STRAINS OF EPSTEIN-BARR-VIRUS
    AITKEN, C
    SENGUPTA, SK
    AEDES, C
    MOSS, DJ
    SCULLEY, TB
    [J]. JOURNAL OF GENERAL VIROLOGY, 1994, 75 : 95 - 100
  • [3] Production of single-stranded DNA using a uracil-N-glycosylase-mediated asymmetric polymerase chain reaction method
    Ball, JK
    Curran, R
    [J]. ANALYTICAL BIOCHEMISTRY, 1997, 253 (02) : 264 - 267
  • [4] SEQUENCE POLYMORPHISM IN THE EPSTEIN-BARR-VIRUS LATENT MEMBRANE-PROTEIN (LMP)-2 GENE
    BUSSON, P
    EDWARDS, RH
    TURSZ, T
    RAABTRAUB, N
    [J]. JOURNAL OF GENERAL VIROLOGY, 1995, 76 : 139 - 145
  • [5] ANALYSIS OF GENOMIC SEQUENCES OF 95 PAPILLOMAVIRUS TYPES - UNITING TYPING, PHYLOGENY, AND TAXONOMY
    CHAN, SY
    DELIUS, H
    HALPERN, AL
    BERNARD, HU
    [J]. JOURNAL OF VIROLOGY, 1995, 69 (05) : 3074 - 3083
  • [6] FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x
  • [7] NUCLEOTIDE-SEQUENCE ANALYSIS OF ALEUTIAN MINK DISEASE PARVOVIRUS SHOWS THAT MULTIPLE VIRUS TYPES ARE PRESENT IN INFECTED MINK
    GOTTSCHALCK, E
    ALEXANDERSEN, S
    COHN, A
    POULSEN, LA
    BLOOM, ME
    AASTED, B
    [J]. JOURNAL OF VIROLOGY, 1991, 65 (08) : 4378 - 4386
  • [8] GOUY M, 1985, COMPUT APPL BIOSCI, V1, P167
  • [9] Sequence variability among-different parvovirus B19 isolates
    Hemauer, A
    vonPoblotzki, A
    Gigler, A
    Cassinotti, P
    Siegl, G
    Wolf, H
    Modrow, S
    [J]. JOURNAL OF GENERAL VIROLOGY, 1996, 77 : 1781 - 1785
  • [10] Höhne M, 1998, J GEN VIROL, V79, P2761