Production of single-stranded DNA using a uracil-N-glycosylase-mediated asymmetric polymerase chain reaction method

被引：8

作者：

Ball, JK

Curran, R

机构：

[1] Div. of Microbiol. and Infect. Dis., Dept. of Clin. Laboratory Sciences, University Hospital, Nottingham

来源：

ANALYTICAL BIOCHEMISTRY | 1997年 / 253卷 / 02期

基金：

英国惠康基金;

关键词：

D O I：

10.1006/abio.1997.2348

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：264 / 267

页数：4

共 11 条

[1] [Anonymous], 1989, SYNTHETIC OLIGONUCLE
[2] INCORPORATION OF SINGLE-STRANDED-DNA BINDING-PROTEIN EARLY IN POLYMERASE CHAIN-REACTION PRODUCT SEQUENCING REACTIONS PREVENTS ENZYME PAUSING
BALL, JK
DESSELBERGER, U
[J]. ANALYTICAL BIOCHEMISTRY, 1992, 207 (02) : 349 - 351
[3] THE USE OF URACIL-N-GLYCOSYLASE IN THE PREPARATION OF PCR PRODUCTS FOR DIRECT SEQUENCING
BALL, JK
DESSELBERGER, U
[J]. NUCLEIC ACIDS RESEARCH, 1992, 20 (12) : 3255 - 3255
[4] DUNCAN BK, 1988, ENZYMES A, P565
[5] DIRECT SOLID-PHASE SEQUENCING OF GENOMIC AND PLASMID DNA USING MAGNETIC BEADS AS SOLID SUPPORT
HULTMAN, T
STAHL, S
HORNES, E
UHLEN, M
[J]. NUCLEIC ACIDS RESEARCH, 1989, 17 (13) : 4937 - 4946
[6] USE OF URACIL DNA GLYCOSYLASE TO CONTROL CARRY-OVER CONTAMINATION IN POLYMERASE CHAIN-REACTIONS
LONGO, MC
BERNINGER, MS
HARTLEY, JL
[J]. GENE, 1990, 93 (01) : 125 - 128
[7] MCCABE PC, 1990, PCR PROTOCOLS GUIDE
[8] PRIMER-DIRECTED ENZYMATIC AMPLIFICATION OF DNA WITH A THERMOSTABLE DNA-POLYMERASE
SAIKI, RK
GELFAND, DH
STOFFEL, S
SCHARF, SJ
HIGUCHI, R
HORN, GT
MULLIS, KB
ERLICH, HA
[J]. SCIENCE, 1988, 239 (4839) : 487 - 491
[9] HUMAN IMMUNODEFICIENCY VIRUS-INFECTED INDIVIDUALS CONTAIN PROVIRUS IN SMALL NUMBERS OF PERIPHERAL MONONUCLEAR-CELLS AND AT LOW COPY NUMBERS
SIMMONDS, P
BALFE, P
PEUTHERER, JF
LUDLAM, CA
BISHOP, JO
BROWN, AJL
[J]. JOURNAL OF VIROLOGY, 1990, 64 (02) : 864 - 872
[10] Heterogeneity of primer extension products in asymmetric PCR is due both to cleavage by a structure-specific exo/endonuclease activity of DNA polymerases and to premature stops
Tombline, G
Bellizzi, D
Sgaramella, V
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (07) : 2724 - 2728

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