Simul 5 - Free dynamic simulator of electrophoresis

被引：168

作者：

Hruska, V ^{[1
]}

Jaros, M ^{[1
]}

Gas, B ^{[1
]}

机构：

[1] Charles Univ, Fac Sci, CZ-12840 Prague 2, Czech Republic

来源：

ELECTROPHORESIS | 2006年 / 27卷 / 5-6期

关键词：

concentration profile; isotachophoresis; numerical solution; partial differential equation; system zone;

D O I：

10.1002/elps.200500756

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We introduce the mathematical model of electromigration of electrolytes in free solution together with free software Simul, version 5, designed for simulation of electrophoresis. The mathematical model is based on principles of mass conservation, acid-base equilibria, and electroneutrality. It accounts for any number of multivalent electrolytes or ampholytes and yields a complete picture about dynamics of electromigration and diffusion in the separation channel. Additionally, the model accounts for the influence of ionic strength on ionic mobilities and electrolyte activities. The typical use of Simul is: inspection of system peaks (zones), stacking and preconcentrating analytes, resonance phenomena, and optimization of separation conditions, in either CZE, ITP, or IEF.

引用

页码：984 / 991

页数：8

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