Investigation of the relationship between the lectin activity of azospirilla and their α-glucosidase, β-glucosidase, and β-galactosidase

被引：1

作者：

Alen'kina, SA ^{[1
]}

Nikitina, VE ^{[1
]}

Borisova-Golovko, MV ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Biochem & Physiol Plants & Microorganisms, Saratov 410015, Russia

来源：

MICROBIOLOGY | 2001年 / 70卷 / 05期

关键词：

azospirilla; alpha-glucosidase; beta-glucosidase; beta-galactosidase; lectin activity;

D O I：

10.1023/A:1012308205568

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The activities of alpha -glucosidase, beta -glucosidase, and 0-galactosidase were studied during the isolation and purification of lectins from Azospirillum brasilense Sp7 and Azospirillum lipoferum 59b cel Is. These enzymatic activities were revealed in crude extracts of surface proteins, protein fraction precipitated with ammonium sulfate or ethanol-acetone mixture, and protein fraction obtained by gel filtration on Sephadex G-75. The distribution of the enzymes between different protein fractions varied for the azospirilla studied. The cofunction of the A. brasilense Sp7 lectin and 0-galactosidase on the cell surface is assumed. A strong interaction between the A. lipoferum 59b lectin and glucosidases was revealed. The lectin from A. lipoferum 59b may possess saccharolytic, activity.

引用

页码：557 / 559

页数：3

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