Evaluation of the New NucliSENS EasyQ KPC Test for Rapid Detection of Klebsiella pneumoniae Carbapenemase Genes (blaKPC)

被引：30

作者：

Spanu, Teresa ^{[1
]}

Fiori, Barbara ^{[1
]}

D'Inzeo, Tiziana ^{[1
]}

Canu, Giulia ^{[1
]}

Campoli, Serena ^{[1
]}

Giani, Tommaso ^{[3
]}

Palucci, Ivana ^{[1
]}

Tumbarello, Mario ^{[2
]}

Sanguinetti, Maurizio ^{[1
]}

Rossolini, Gian Maria ^{[3
,4
]}

机构：

[1] Univ Cattolica Sacro Cuore, Inst Microbiol, Agostino Gemelli Hosp, I-00168 Rome, Italy

[2] Univ Cattolica Sacro Cuore, Inst Infect Dis, Agostino Gemelli Hosp, I-00168 Rome, Italy

[3] Univ Siena, Microbiol Sect, Dept Biotechnol, I-53100 Siena, Italy

[4] Siena Univ Hosp, Clin Microbiol & Virol Unit, Siena, Italy

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 08期

关键词：

METALLO-BETA-LACTAMASES; CTX-M; DNA MICROARRAY; SHV; RESISTANCE; OUTBREAK; ASSAY; TEM;

D O I：

10.1128/JCM.00284-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

KPC-type carbapenemases are emerging in Klebsiella pneumoniae and other Gram-negative pathogens worldwide. Rapid and sensitive detection of these resistance determinants has become relevant to clinical management and infection control. We evaluated the bioMerieux EasyQ real-time PCR assay for bla(KPC) detection with 300 members of the Enterobacteriaceae, including 29 control strains producing known carbapenemases and 271 nonreplicate clinical isolates. The EasyQ assay correctly detected all of the 111 isolates harboring bla(KPC) genes, with no false positives, and results were available within 2 h.

引用

页码：2783 / 2785

页数：3

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