Neurochemical characterization of neurons expressing melanin-concentrating hormone receptor 1 in the mouse hypothalamus

被引:69
作者
Chee, Melissa J. S. [1 ]
Pissios, Pavlos [1 ]
Maratos-Flier, Eleftheria [1 ]
机构
[1] Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Endocrinol, Boston, MA USA
基金
美国国家卫生研究院;
关键词
Cre recombinase; tdTomato; slc-1; immunohistochemistry; leptin; GPR24; THYROTROPIN-RELEASING-HORMONE; MESOLIMBIC DOPAMINE SYSTEM; MESSENGER-RNA EXPRESSION; RAT LATERAL HYPOTHALAMUS; CENTRAL-NERVOUS-SYSTEM; DIET-INDUCED OBESITY; BODY-WEIGHT; GLUCOSE-HOMEOSTASIS; ARCUATE NUCLEUS; POMC NEURONS;
D O I
10.1002/cne.23273
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Melanin-concentrating hormone (MCH) is a hypothalamic neuropeptide that acts via MCH receptor 1 (MCHR1) in the mouse. It promotes positive energy balance; thus, mice lacking MCH or MCHR1 are lean, hyperactive, and resistant to diet-induced obesity. Identifying the cellular targets of MCH is an important step to understanding the mechanisms underlying MCH actions. We generated the Mchr1-cre mouse that expresses cre recombinase driven by the MCHR1 promoter and crossed it with a tdTomato reporter mouse. The resulting Mchr1-cre/tdTomato progeny expressed easily detectable tdTomato fluorescence in MCHR1 neurons, which were found throughout the olfactory system, striatum, and hypothalamus. To chemically identify MCH-targeted cell populations that play a role in energy balance, MCHR1 hypothalamic neurons were characterized by colabeling select hypothalamic neuropeptides with tdTomato fluorescence. TdTomato fluorescence colocalized with dynorphin, oxytocin, vasopressin, enkephalin, thyrothropin-releasing hormone, and corticotropin-releasing factor immunoreactive cells in the paraventricular nucleus. In the lateral hypothalamus, neurotensin, but neither orexin nor MCH neurons, expressed tdTomato. In the arcuate nucleus, both Neuropeptide Y and proopiomelanocortin cells expressed tdTomato. We further demonstrated that some of these arcuate neurons were also targets of leptin action. Interestingly, MCHR1 was expressed in the vast majority of leptin-sensitive proopiomelanocortin neurons, highlighting their importance for the orexigenic actions of MCH. Taken together, this study supports the use of the Mchr1-cre mouse for outlining the neuroanatomical distribution and neurochemical phenotype of MCHR1 neurons. J. Comp. Neurol. 521:22082234, 2013. (c) 2012 Wiley Periodicals, Inc.
引用
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页码:2208 / 2234
页数:27
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