Linear phase imaging using differential interference contrast microscopy

被引：229

作者：

Arnison, MR

Larkin, KG

Sheppard, CJR

Smith, NI

Cogswell, CJ

机构：

[1] Canon Informat Syst Res Australia, N Ryde, NSW 2113, Australia

[2] Univ Sydney, Sch Phys, Phys Opt Lab, Sydney, NSW 2006, Australia

[3] Dept Appl Phys, Suita, Osaka 5650871, Japan

[4] Univ Colorado, Dept Elect & Comp Engn, Boulder, CO 80309 USA

来源：

JOURNAL OF MICROSCOPY-OXFORD | 2004年 / 214卷 / 01期

关键词：

differential interference contrast microscopy; Fourier transform; Hilbert transform; image processing; Nomarski differential interference contrast; phase retrieval; phase shifting; spiral phase transform;

D O I：

10.1111/j.0022-2720.2004.01293.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

We propose an extension to Nomarski differential interference contrast microscopy that enables isotropic linear phase imaging. The method combines phase shifting, two directions of shear and Fourier-space integration using a modified spiral phase transform. We simulated the method using a phantom object with spatially varying amplitude and phase. Simulated results show good agreement between the final phase image and the object phase, and demonstrate resistance to imaging noise.

引用

页码：7 / 12

页数：6

共 27 条

[1] Using the Hilbert transform for 3D visualization of differential interference contrast microscope images [J].