Lymphostin (LK6-A), a novel immunosuppressant from Streptomyces sp. KY11783: Taxonomy of the producing organism, fermentation, isolation and biological activities

被引：46

作者：

Nagata, H

Ochiai, K

Aotani, Y

Ando, K

Yoshida, M

Takahashi, I

Tamaoki, T

机构：

[1] Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Machida-shi, Tokyo 194

[2] Pharmaceutical Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Sunto, Shizuoka 411

来源：

JOURNAL OF ANTIBIOTICS | 1997年 / 50卷 / 07期

关键词：

D O I：

10.7164/antibiotics.50.537

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In the course of screening for inhibitors of the lymphocyte kinase, Lck (p56(lck)), aiming at novel immunosuppressants, we isolated a novel alkaloid, lymphostin (LK6-A), from the culture broth of Streptomyces sp. KY11783. Lymphostin was produced in a fermentation medium supplemented with a highly porous polymer resin, which prevented the degradation of this compound in the culture broth. Lymphostin inhibited the kinase activity of Lck with an IC50 value of 0.05 mu M, and exhibited potent inhibitory activity against the mixed lymphocyte reaction (MLR) with an IC50 value of 0.009 mu M.

引用

收藏

页码：537 / 542

页数：6

相关论文

共 25 条

[1] INVOLVEMENT OF THE PROTEIN-TYROSINE KINASE P56(LCK) IN T-CELL SIGNALING AND THYMOCYTE DEVELOPMENT [J].

ANDERSON, SJ ;

LEVIN, SD ;

PERLMUTTER, RM .

ADVANCES IN IMMUNOLOGY, VOLUME 56, 1994, 56 :151-178

[2] Lymphostin (LK6-A), a novel immunosuppressant from Streptomyces sp. KY11783: Structural elucidation [J].

Aotani, Y ;

Nagata, H ;

Yoshida, M .

JOURNAL OF ANTIBIOTICS, 1997, 50 (07) :543-545

[3] USE OF SYNTHETIC PEPTIDES AND COPOLYMERS TO STUDY THE SUBSTRATE-SPECIFICITY AND INHIBITION OF THE PROTEIN-TYROSINE KINASE PP60(C-SRC) [J].

BUDDE, RJA ;

OBEYESEKERE, NU ;

KE, S ;

MCMURRAY, JS .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1995, 1248 (01) :50-56

[4] BICYCLIC COMPOUNDS AS RING-CONSTRAINED INHIBITORS OF PROTEIN-TYROSINE KINASE-P56LCK [J].

BURKE, TR ;

LIM, B ;

MARQUEZ, VE ;

LI, ZH ;

BOLEN, JB ;

STEFANOVA, I ;

HORAK, ID .

JOURNAL OF MEDICINAL CHEMISTRY, 1993, 36 (04) :425-432

[5] SYNTHESIS AND PROTEIN-TYROSINE KINASE INHIBITORY ACTIVITIES OF FLAVONOID ANALOGS [J].

CUSHMAN, M ;

NAGARATHNAM, D ;

BURG, DL ;

GEAHLEN, RL .

JOURNAL OF MEDICINAL CHEMISTRY, 1991, 34 (02) :798-806

[6] Discovery of a novel, potent, and Src family-selective tyrosine kinase inhibitor - Study of Lck- and FynT-dependent T cell activation [J].

Hanke, JH ;

Gardner, JP ;

Dow, RL ;

Changelian, PS ;

Brissette, WH ;

Weringer, EJ ;

Pollok, K ;

Connelly, PA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (02) :695-701

[7] A RAPID ANALYSIS FOR CHEMICAL GROUPING OF AEROBIC ACTINOMYCETES [J].

HASEGAWA, T ;

TAKIZAWA, M ;

TANIDA, S .

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 1983, 29 (04) :319-322

[8] IMMUNOSUPPRESSIVE EFFECT OF FK506 ON COLLAGEN-INDUCED ARTHRITIS IN RATS [J].

INAMURA, N ;

HASHIMOTO, M ;

NAKAHARA, K ;

AOKI, H ;

YAMAGUCHI, I ;

KOHSAKA, M .

CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, 1988, 46 (01) :82-90

[9] Sequential Interactions of the TCR with Two Distinct Cytoplasmic Tyrosine Kinases [J].

Iwashima, Makio ;

Irving, Bryan A. ;

van Oers, Nicolai S. C. ;

Chan, Andrew C. ;

Weiss, Arthur .

JOURNAL OF IMMUNOLOGY, 2014, 193 (09) :4279-4282

[10]

KAWASHIMA H, 1988, INVEST OPHTH VIS SCI, V29, P1265