A leukocyte cryopreservation technique for cytogenetic studies

被引：13

作者：

Duarte, JMB ^{[1
]}

Ramalho, MFPD

de Lima, VFH

Jorge, W

机构：

[1] UNESP, FCAV, Dept Melhoramento Genet Anim, BR-14870000 Jaboticabal, SP, Brazil

[2] UNESP, FCAV, Dept Reproducao Anim, BR-14870000 Jaboticabal, SP, Brazil

[3] UFMG, ICB, Inst Biol Geral, BR-30161970 Belo Horizonte, MG, Brazil

来源：

GENETICS AND MOLECULAR BIOLOGY | 1999年 / 22卷 / 03期

关键词：

D O I：

10.1590/S1415-47571999000300019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several culture, cryopreservation, freezing and thawing methods were tested to develop an efficient technique for storing cervid and ovine leukocytes. The best results were obtained with McCoy or Vega y Martinet (VYM) solution with dimethyl sulfoxide (DMSO), horse serum and polyvinylpyrrolidone (PVP) as cryopreservatives. The best protocol for freezing was 4 degrees C for 30 min followed by 15 min in liquid nitrogen vapor. To thaw, the still frozen material was placed onto cultivation medium for propagation.

引用

页码：399 / 400

页数：2

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