Δ9-Tetrahydrocannabinol induces apoptosis in human prostate PC-3 cells via a receptor-independent mechanism

被引：140

作者：

Ruiz, L ^{[1
]}

Miguel, A ^{[1
]}

Díaz-Laviada, I ^{[1
]}

机构：

[1] Univ Alcala de Henares, Fac Med, Dept Biochem & Mol Biol, Alcala De Henares 28871, Madrid, Spain

来源：

FEBS LETTERS | 1999年 / 458卷 / 03期

关键词：

cannabinoid; cannabinoid receptor; apoptosis; prostate cell;

D O I：

10.1016/S0014-5793(99)01073-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of Delta(9)-tetrahydrocannabinol (THC), the major psycho-active component of marijuana, in human prostate cancer cells PC-3 was investigated. THC caused apoptosis in a dose-dependent manner, Morphological and biochemical changes induced by THC in prostate PC-3 cells shared the characteristics of an apoptotic phenomenon. First, loss of plasma membrane asymmetry determined by fluorescent anexin V binding. Second, presence of apoptotic bodies and nuclear fragmentation observed by DNA staining with 4',6-diamino-2-phenylindole (DAPI), Third, presence of typical 'ladder-patterned' DNA fragmentation. Central cannabinoid receptor expression vc;as observed in PC-3 cells by immunofluorescence studies, However. several results indicated that the apoptotic effect,vas cannabinoid receptor-independent, such as lack of an effect of the potent cannabinoid agonist WIN 55,212-2, inability of cannabinoid antagonist AM 251 to prevent cellular death caused by THC and absence of an effect of pertussis toxin pre-treatment. (C) 1999 Federation of European Biochemical Societies.

引用

页码：400 / 404

页数：5

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