Spatially well-defined binary brushes of poly(ethylene glycol)s for micropatterning of active proteins on anti-fouling surfaces

被引：52

作者：

Xu, F. J. ^{[1
,2
]}

Li, H. Z. ^{[3
]}

Li, J. ^{[2
,3
]}

Teo, Y. H. Eric ^{[4
]}

Zhu, C. X. ^{[4
]}

Kang, E. T. ^{[1
]}

Neoh, K. G. ^{[1
]}

机构：

[1] Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 119260, Singapore

[2] Natl Univ Singapore, Div Bioengn, Singapore 117602, Singapore

[3] Inst Mat Res & Engn, Singapore 117602, Singapore

[4] Natl Univ Singapore, Dept Elect & Comp Engn, Singapore 119260, Singapore

来源：

BIOSENSORS & BIOELECTRONICS | 2008年 / 24卷 / 04期

关键词：

Micropatterning; Protein; Biosensor; ATRP; PEG;

D O I：

10.1016/j.bios.2008.06.055

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We report a novel method for micropatterning of active proteins on anti-fouling surfaces via spatially well-defined and dense binary poly(ethylene glycol)s (PEGs) brushes with controllable protein-docking sites. Binary brushes of poly(poly(ethylene glycol) methacrylate-co-poly(ethylene glycol)methyl ether methacrylate), or P(PEGMA-co-PEGMEMA), and poly(poly(ethylene glycol)methyl ether methacrylate), or P(PEGMEMA), were prepared via consecutive surface-initiated atom transfer radical polymerizations (SI-ATRPs) from a resist-micro patterned Si(I 0 0) wafer surface. The terminal hydroxyl groups on the side chains of PEGMA units in the P(PEGMA-co-PEGMEMA) microdomains were activated directly by 1,1'-carbonyldiimidazole (CDI) for the covalent coupling of human immunoglobulin (IgG) (as a model active protein). The resulting IgG-coupled PEG microdomains interact only and specifically with target anti-IgG, while the other PEG microregions effectively prevent specific and non-specific protein fouling. When extended to other active biomolecules, microarrays for specific and non-specific analyte interactions with a high signal-to-noise ratio could be readily tailored. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：773 / 780

页数：8

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