Desiccation and rehydration elicit distinct heat shock protein transcript responses in flesh fly pupae

被引:99
作者
Hayward, SAL [1 ]
Rinehart, JP [1 ]
Denlinger, DL [1 ]
机构
[1] Ohio State Univ, Dept Entomol, Columbus, OH 43210 USA
关键词
diapause; insect; Sarcophaga crassipalpis; stress; water loss; heat shock protein; flesh fly; desiccation; rehydration;
D O I
10.1242/jeb.00842
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Heat shock proteins (Hsps) are a ubiquitous component of the cellular response to stress in both prokaryotic and eukaryotic organisms, but their role and function during desiccation stress in terrestrial arthropods has received limited attention. Molecular responses to rehydration are arguably as important as those to desiccation in maintaining cellular integrity and enzyme activity, but the role of Hsps during stress recovery is poorly understood and has never been addressed with respect to rehydration in insects. This study identifies distinct differences in the Hsp response to desiccation and rehydration in the flesh fly Sarcophaga crassipalpis, as well as differences in the desiccation responses of diapausing and nondiapausing pupae. In nondiapausing pupae, the expression of two inducible Hsps (Hsp23 and Hsp70) is upregulated by desiccation, but the water loss threshold for Hsp expression changes at different rates of dehydration. Continued desiccation results in the prolonged expression of both Hsp23 and Hsp70, which may contribute to the delayed adult eclosion noted in samples desiccated for more than 3 days at <5% relative humidity/25degreesC. In diapausing pupae, hsp23 and hsp70 transcripts are already highly expressed and are not further upregulated by desiccation stress. Both of the constitutive Hsps investigated, Hsp90 and Hsc70, were unresponsive to desiccation in both nondiapausing and diapausing pupae. However, both Hsp90 and Hsc70 were upregulated upon rehydration in nondiapausing and diapausing pupae. These results indicate distinct roles for the different Hsps during desiccation stress and rehydration/stress recovery. The response to desiccation recovery (rehydration) is similar to the Hsp response to cold recovery identified in S. crassipalpis: Hsp90 and Hsc70 are upregulated in both cases.
引用
收藏
页码:963 / 971
页数:9
相关论文
共 52 条
[41]   Desiccation elicits heat shock protein transcription in the flesh fly, Sarcophaga crassipalpis, but does not enhance tolerance to high or low temperatures [J].
Tammariello, SP ;
Rinehart, JP ;
Denlinger, DL .
JOURNAL OF INSECT PHYSIOLOGY, 1999, 45 (10) :933-938
[42]   Small heat-shock proteins regulate membrane lipid polymorphism [J].
Tsvetkova, NM ;
Horváth, I ;
Török, Z ;
Wolkers, WF ;
Balogi, Z ;
Shigapova, N ;
Crowe, LM ;
Tablin, F ;
Vierling, E ;
Crowe, JH ;
Vigh, L .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (21) :13504-13509
[43]   HSP70-mediated acceleration of translational recovery after stress is independent of ribosomal RNA synthesis [J].
Van Nieuwenhoven, FA ;
Martin, X ;
Heijnen, VVT ;
Cornelussen, RN ;
Snoeckx, LHEH .
EUROPEAN JOURNAL OF CELL BIOLOGY, 2001, 80 (09) :586-592
[44]  
Viner RI, 2001, CELL STRESS CHAPERON, V6, P126, DOI 10.1379/1466-1268(2001)006<0126:IOTOTM>2.0.CO
[45]  
2
[46]   ALTERATION OF THE ECLOSION RHYTHM AND ECLOSION BEHAVIOR IN THE FLESH FLY, SARCOPHAGA-CRASSIPALPIS, BY LOW AND HIGH-TEMPERATURE STRESS [J].
YOCUM, GD ;
ZDAREK, J ;
JOPLIN, KH ;
LEE, RE ;
SMITH, DC ;
MANTER, KD ;
DENLINGER, DL .
JOURNAL OF INSECT PHYSIOLOGY, 1994, 40 (01) :13-21
[47]   Differential expression of two HSP70 transcripts in response to cold shock, thermoperiod, and adult diapause in the Colorado potato beetle [J].
Yocum, GD .
JOURNAL OF INSECT PHYSIOLOGY, 2001, 47 (10) :1139-1145
[48]   Upregulation of a 23 kDa small heat shock protein transcript during pupal diapause in the flesh fly, Sarcophaga crassipalpis [J].
Yocum, GD ;
Joplin, KH ;
Denlinger, DL .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1998, 28 (09) :677-682
[49]  
YODER JA, 1991, J EXP BIOL, V157, P273
[50]   ENHANCEMENT OF DIAPAUSING FLESH FLY PUPARIA WITH ADDITIONAL HYDROCARBONS AND EVIDENCE FOR ALKANE BIOSYNTHESIS BY A DECARBONYLATION MECHANISM [J].
YODER, JA ;
DENLINGER, DL ;
DENNIS, MW ;
KOLATTUKUDY, PE .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1992, 22 (03) :237-243