Mass Spectrometry Based Targeted Protein Quantification: Methods and Applications

被引：267

作者：

Pan, Sheng ^{[1
]}

Aebersold, Ruedi ^{[2
,3
,4
]}

Chen, Ru ^{[5
]}

Rush, John

Goodlett, David R. ^{[6
]}

McIntosh, Martin W. ^{[7
]}

Zhang, Jing ^{[1
]}

Brentnall, Teresa A. ^{[5
]}

机构：

[1] Univ Washington, Dept Pathol, Seattle, WA 98195 USA

[2] Univ Zurich, Fac Sci, CH-8006 Zurich, Switzerland

[3] ETH, Inst Mol Syst Biol, Zurich, Switzerland

[4] Inst Syst Biol, Seattle, WA 98103 USA

[5] Univ Washington, Div Gastroenterol, Dept Med, Seattle, WA 98195 USA

[6] Univ Washington, Dept Med Chem, Seattle, WA 98195 USA

[7] Fred Hutchinson Canc Res Ctr, Seattle, WA 98109 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2009年 / 8卷 / 02期

基金：

美国国家卫生研究院; 瑞士国家科学基金会;

关键词：

proteomics; absolute quantification; mass spectrometry; biomarker; MRM; MALDI TOF/TOF; AQUA; SISCAPA; stable isotope dilution; MULTIPLEXED ABSOLUTE QUANTIFICATION; CONCATENATED SIGNATURE PEPTIDES; REACTION MONITORING ASSAYS; LOW-ABUNDANCE PROTEINS; DIRECT TISSUE-ANALYSIS; N-TERMINAL PEPTIDES; BIOMARKER DISCOVERY; ISOTOPE-DILUTION; LIQUID-CHROMATOGRAPHY; QUANTITATIVE-ANALYSIS;

D O I：

10.1021/pr800538n

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The recent advance in technology for mass spectrometry-based targeted protein quantification has opened new avenues for a broad range of proteomic applications in clinical research. The major breakthroughs are highlighted by the capability of using a "universal" approach to perform quantitative assays for a wide spectrum of proteins with minimum restrictions and the ease of assembling multiplex detections in a single measurement. The quantitative approach relies on the use of synthetic stable isotope labeled peptides or proteins, which precisely mimic their endogenous counterparts and act as internal standards to quantify the corresponding candidate proteins. This report reviews recently developed platform technologies for emerging applications of clinical proteomics and biomarker development.

引用

页码：787 / 797

页数：11

共 111 条

[1] Coupling immunoaffinity techniques with MS for quantitative analysis of low-abundance protein biomarkers [J].