A comparative physical study of two different hydrophilic synthetic latex matrices for the construction of a glucose biosensor

被引:37
作者
Cosnier, S
Szunerits, S
Marks, RS
Novoa, A
Puech, L
Perez, E
Rico-Lattes, I
机构
[1] Univ Grenoble 1, CNRS, Lab Electrochim Organ & Photochim Redox, UMR 5630, F-38041 Grenoble 9, France
[2] Ben Gurion Univ Negev, Inst Appl Biosci, IL-84105 Beer Sheva, Israel
[3] Ben Gurion Univ Negev, Dept Biotechnol Engn, IL-84105 Beer Sheva, Israel
[4] Univ Toulouse 3, Lab IMRCP, CNRS, UMR 5623, F-31062 Toulouse, France
关键词
latex; glucose oxidase; biosensor; enzyme electrode; permeability;
D O I
10.1016/S0039-9140(01)00498-2
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Two different biodegradable latex polymers functionalised by hydroxy (1) or gluconamide (2) groups proved to be good immobilisation matrixes for glucose oxidase. The responses of these biosensors to glucose additions were measured by potentiostating the modified electrodes at 0.6 V/SCE in order to oxidise the hydrogen peroxide generated by the enzymatic oxidation of glucose in the presence of oxygen. The response of such electrodes was evaluated as a function of film thickness, pH and temperature. Rotating disk electrode experiments showed the influence of the enzyme on the structure of both latex films, namely a marked improvement in matrix permeability. The high permeability of the latex 1 based enzyme sensor (bilayer, P-m = 8.10 x 10(-4) cm s(-1)) resulted in a high dynamic range. Furthermore, the activation energy for a latex 1 sensor was determined to be 44.55 and 18.03 kJ mol(-1), respectively depending on the conformation of the enzyme. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:889 / 897
页数:9
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