ELECTROCHEMICAL IMMOBILIZATION OF GLUCOSE-OXIDASE IN POLY(AMPHIPHILIC PYRROLE) FILMS AND ITS APPLICATION TO THE PREPARATION OF AN AMPEROMETRIC GLUCOSE SENSOR

A glucose sensor has been realized by the electropolymerization on a platinum electrode of a pre-adsorbed coating of glucose oxidase (GOD) mixed with the amphiphilic substituted pyrrole 1. In the presence of glucose and molecular oxygen, this biosensor has a fast response time and provides a low detection limit (0.1 mu M). The stability, pH, temperature response of the sensor and its kinetic parameters have been studied. The preparation procedure enables the control of the amount and the activity of entrapped enzyme. Multilayered assemblies could be easily obtained by sequential electropolymerization. These structures allowed to increase notably the linear part of the calibration curves, from 2.5 to 5 mM in glucose, and to minimize the electrochemical interferences due to endogenous electroactive substances. Preliminary attempts at the preparation of miniature glucose sensors have demonstrated the potential interest of this new biosensor construction technique.