Multiple Separations in Microfabricated Channels: From Biological Microenvironments to DNA

被引：6

作者：

Bullard, Katherine M. ^{[1
]}

Hietpas, Paula Beyer ^{[1
]}

Ewing, Andrew G. ^{[1
]}

机构：

[1] Penn State Univ, Dept Chem, University Pk, PA 16802 USA

来源：

BIOMEDICAL MICRODEVICES | 1998年 / 1卷 / 01期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

continuous separations; microfabricated; channel electrophoresis; review; DNA;

D O I：

10.1023/A:1009926205209

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A continuous sample introduction and separation scheme is presented as an alternative to the current slab gel and microfabricated chip technologies for biological separations. This new technique involves continuous sample introduction via a conventional small bore capillary coupled to a small dimension rectangular channel. Both free zone and size based separations have been carried out in the rectangular channel. Laser induced fluorescence and electrochemical detection schemes have been employed with this technique. Some of the areas this technology has been used to investigate include monitoring dynamic events from microenvironments, monitoring analytes over long periods of time, and performing DNA separations.

引用

页码：27 / 37

页数：11

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