MiniX-STR multiplex system population study in Japan and application to degraded DNA analysis

被引:55
作者
Asamura, H [1 ]
Sakai, H [1 ]
Kobayashi, K [1 ]
Ota, M [1 ]
Fukushima, H [1 ]
机构
[1] Shinshu Univ, Sch Med, Dept Legal Med, Nagano 3908621, Japan
关键词
X chromosome; short tandem repeat (STR); smaller amplicon lengths; multiplex systems; degraded DNA;
D O I
10.1007/s00414-005-0074-6
中图分类号
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号
0301 ; 10 ;
摘要
We sought to evaluate a more effective system for analyzing X-chromosomal short tandem repeats (X-STRs) in highly degraded DNA. To generate smaller amplicon lengths, we designed new polymerase chain reaction (PCR) primers for DXS7423, DXS6789, DXS101, GATA31E08, DXS8378, DXS7133, DXS7424, and GATA165B12 at X-linked short tandem repeat (STR) loci, devising two miniX-multiplex PCR systems. Among 333 Japanese individuals, these X-linked loci were detected in amplification products ranging in length from 76 to 169 bp, and statistical analyses of the eight loci indicated a high usefulness for the Japanese forensic practice. Results of tests on highly degraded DNA indicated the miniX-STR multiplex strategies to be an effective system for analyzing degraded DNA. We conclude that analysis by the current miniX-STR multiplex systems offers high effectiveness for personal identification from degraded DNA samples.
引用
收藏
页码:174 / 181
页数:8
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