Effect of excitation wavelength on penetration depth in nonlinear optical microscopy of turbid media

被引：82

作者：

Balu, Mihaela ^{[1
]}

Baldacchini, Tommaso ^{[2
]}

Carter, John ^{[2
]}

Krasieva, Tatiana B. ^{[1
]}

Zadoyan, Ruben ^{[2
]}

Tromberg, Bruce J. ^{[1
]}

机构：

[1] Univ Calif Irvine, Beckman Laser Inst, Laser Microbeam & Med Program, Irvine, CA 92617 USA

[2] Newport Corp, Technol & Applicat Ctr, Irvine, CA 92606 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2009年 / 14卷 / 01期

关键词：

two-photon excited fluorescence; second harmonic generation; nonlinear optical microscopy;

D O I：

10.1117/1.3081544

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a comparative study of two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) imaging in turbid media at 800- and 1300-nm excitation. The depth-dependent decay of TPEF and SHG signals in turbid tissue phantoms is used to estimate the impact of light scattering on excitation intensity at each wavelength. A 50 to 80% increase in scattering length is observed using 1300-nm excitation, while peak TPEF emission intensity is obtained 10 to 20 mu m beneath the surface for both sources. The increased penetration depth at 1300 nm is confirmed by TPEF and SHG microscopy of tissue phantoms composed of gelatin/microspheres and 3-D organotypic collagen-fibroblast cultures, respectively. Our results establish the feasibility of 1.3-mu m excitation in nonlinear optical microscopy. (c) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3081544]

引用

页数：3

共 9 条

[1] Epifluorescence collection in two-photon microscopy [J].

Beaurepaire, E ;

Mertz, J .

APPLIED OPTICS, 2002, 41 (25) :5376-5382

[2] Photodynamic therapy on keloid fibroblasts in tissue-engineered keratinocyte-fibroblast co-culture [J].

Chiu, LL ;

Sun, CH ;

Yeh, AT ;

Torkian, B ;

Karamzadeh, A ;

Tromberg, B ;

Wong, BJF .

LASERS IN SURGERY AND MEDICINE, 2005, 37 (03) :231-244

[3] Multimodal nonlinear spectral microscopy based on a femtosecond Cr:forsterite laser [J].

Chu, SW ;

Chen, IH ;

Liu, TM ;

Chen, PC ;

Sun, CK ;

Lin, BL .

OPTICS LETTERS, 2001, 26 (23) :1909-1911

[4] Influence of optical properties on two-photon fluorescence imaging in turbid samples [J].

Dunn, AK ;

Wallace, VP ;

Coleno, M ;

Berns, MW ;

Tromberg, BJ .

APPLIED OPTICS, 2000, 39 (07) :1194-1201

[5] MPScope: A versatile software suite for multiphoton microscopy [J].

Nguyen, Quoc-Thang ;

Tsai, Philbert S. ;

Kleinfeld, David .

JOURNAL OF NEUROSCIENCE METHODS, 2006, 156 (1-2) :351-359

[6] Two-photon microscopy in brain tissue: parameters influencing the imaging depth (vol 111, pg 29, 2001) [J].

Oheim, M ;

Beaurepaire, E ;

Chaigneau, E ;

Mertz, J ;

Charpak, S .

JOURNAL OF NEUROSCIENCE METHODS, 2001, 112 (02) :205-205

[7]

TANG S, 2006, J BIOMED OPT, V11, P20503

[8] Two-photon imaging to a depth of 1000 μm in living brains by use of a Ti:Al2O3 regenerative amplifier [J].

Theer, P ;

Hasan, MT ;

Denk, W .

OPTICS LETTERS, 2003, 28 (12) :1022-1024

[9]

Tromberg BJ., 2008, Handbook of Biomedical Nonlinear Optical Microscopy, P707

← 1 →