Two-photon imaging to a depth of 1000 μm in living brains by use of a Ti:Al2O3 regenerative amplifier

被引：513

作者：

Theer, P ^{[1
]}

Hasan, MT ^{[1
]}

Denk, W ^{[1
]}

机构：

[1] Max Planck Inst Med Res, D-69120 Heidelberg, Germany

来源：

OPTICS LETTERS | 2003年 / 28卷 / 12期

关键词：

D O I：

10.1364/OL.28.001022

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

It is shown that two-photon fluorescence images can be obtained throughout almost the entire gray matter of the mouse neocortex by using optically amplified femtosecond pulses. The achieved imaging depth approaches the theoretical limit set by excitation of out-of-focus fluorescence. (C) 2003 Optical Society of America.

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页码：1022 / 1024

页数：3

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