Evaluation of a new meropenem-EDTA double-ended Etest strip for the detection of the cfiA metallo-β-lactamase gene in clinical isolates of Bacteroides fragilis

被引：11

作者：

Bogaerts, P. ^{[1
]}

Engelhardt, A. ^{[2
]}

Berhin, C. ^{[1
]}

Bylund, L. ^{[2
]}

Ho, P. ^{[2
]}

Yusof, A. ^{[2
]}

Glupczynski, Y. ^{[1
]}

机构：

[1] Univ Catholique Louvain, Bacteriol Lab, UCL Godinne, Yvoir, Belgium

[2] AB BIODISK, Solna, Sweden

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2008年 / 14卷 / 10期

关键词：

bacteroides; Etest; metallo-beta-lactamase; phenotypic detection;

D O I：

10.1111/j.1469-0691.2008.02065.x

中图分类号：

R51 [传染病];

学科分类号：

100401 [流行病与卫生统计学];

摘要：

Thirty-five Bacteroides fragilis clinical isolates with varying susceptibility to meropenem were analysed with a prototype of a double-ended Etest strip containing meropenem +/- EDTA, designed for the detection of the CfiA metallo-beta-lactamase. Phenotypic results obtained with this new Etest strip were related to the genotype and compared to the results of the Etest containing imipenem +/- EDTA. Whereas the Etest with imipenem +/- EDTA only allowed detection of isolates with high-level resistance (both MICs of imipenem and meropenem > 32 mg/L), reflecting the possible underestimation of CfiA prevalence in B. fragilis, the Etest with meropenem +/- EDTA proved to be more accurate, particularly for isolates with low-level carbapenem resistance, suggesting its potential for broader detection of CfiA production.

引用

页码：973 / 977

页数：6

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