The APRR1/TOC1 quintet implicated in circadian rhythms of Arabidopsis thaliana:: 1.: Characterization with APRR1-overexpressing plants

被引:180
作者
Makino, S [1 ]
Matsushika, A [1 ]
Kojima, M [1 ]
Yamashino, T [1 ]
Mizuno, T [1 ]
机构
[1] Nagoya Univ, Sch Agr, Mol Microbiol Lab, Chikusa Ku, Nagoya, Aichi 4648601, Japan
关键词
Arabidopsis; circadian rhythm; clock components; transgenic plants;
D O I
10.1093/pcp/pcf005
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Several Arabidopsis genes have been proposed to encode potential clock-associated components, including the Myb-related CCA1 and LHY transcription factors and a member of the novel family of pseudo response regulators (APRR1/TOC1). We previously showed that mRNAs of the APRR1/TOC1 family of genes start accumulating after dawn rhythmically and sequentially at approximately 2 h intervals in the order: APRR9-->APRR7-->APRR5-->APRR3 -->APRR1/TOC1. Here we constructed APRR1-overexpressing (APRR1-ox) plants, and examined certain circadian profiles for APRRs, CCA1, LHY, GI, CCR2, and CAB2. The free-running circadian rhythms of the APRR1/TOC1 family of genes, including APRR1, were dampened in APRR1-ox plants. In particular, the light-inducible expression of APRR9 was severely repressed in APRR1-ox plants, suggesting that there is a negative APRR1-->APRR9 regulation. The free-running robust rhythm of CAB2 was also dampened in APRR1-ox. The circadian profiles of potential clock-associated genes, CCA1, LHY, GI, and CCR2 were all markedly altered in APRR1-ox, each in characteristic fashion. To gain further insight into the molecular function of APRR1, we then identified a novel Myc-related bHLH transcription factor, which physically associated with APRR1. This protein (named PIL1) is similar in its amino acid sequence to PIF3, which has been identified as a phytochrome-interacting transcription factor. These results are discussed in relation to the current idea that APRR1 (TOC1) plays a role within, or close to, the Arabidopsis central oscillator.
引用
收藏
页码:58 / 69
页数:12
相关论文
共 51 条
  • [11] Orchestrated transcription of key pathways in Arabidopsis by the circadian clock
    Harmer, SL
    Hogenesch, LB
    Straume, M
    Chang, HS
    Han, B
    Zhu, T
    Wang, X
    Kreps, JA
    Kay, SA
    [J]. SCIENCE, 2000, 290 (5499) : 2110 - 2113
  • [12] AtGRP7, a nuclear RNA-binding protein as a component of a circadian-regulated negative feedback loop in Arabidopsis thaliana
    Heintzen, C
    Nater, M
    Apel, K
    Staiger, D
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (16) : 8515 - 8520
  • [13] Conditional circadian dysfunction of the Arabidopsis early-flowering 3 mutant
    Hicks, KA
    Millar, AJ
    Carre, IA
    Somers, DE
    Straume, M
    MeeksWagner, DR
    Kay, SA
    [J]. SCIENCE, 1996, 274 (5288) : 790 - 792
  • [14] EARLY FLOWERING3 encodes a novel protein that regulates circadian clock function and flowering in Arabidopsis
    Hicks, KA
    Albertson, TM
    Wagner, DR
    [J]. PLANT CELL, 2001, 13 (06) : 1281 - 1292
  • [15] GIGANTEA is a nuclear protein involved in phytochrome signaling in Arabidopsis
    Huq, E
    Tepperman, JM
    Quail, PH
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (17) : 9789 - 9794
  • [16] Compilation and characterization of Arabidopsis thaliana response regulators implicated in His-Asp phosphorelay signal transduction
    Imamura, A
    Hanaki, N
    Nakamura, A
    Suzuki, T
    Taniguchi, M
    Kiba, T
    Ueguchi, C
    Sugiyama, T
    Mizuno, T
    [J]. PLANT AND CELL PHYSIOLOGY, 1999, 40 (07) : 733 - 742
  • [17] Analysis of the genome sequence of the flowering plant Arabidopsis thaliana
    Kaul, S
    Koo, HL
    Jenkins, J
    Rizzo, M
    Rooney, T
    Tallon, LJ
    Feldblyum, T
    Nierman, W
    Benito, MI
    Lin, XY
    Town, CD
    Venter, JC
    Fraser, CM
    Tabata, S
    Nakamura, Y
    Kaneko, T
    Sato, S
    Asamizu, E
    Kato, T
    Kotani, H
    Sasamoto, S
    Ecker, JR
    Theologis, A
    Federspiel, NA
    Palm, CJ
    Osborne, BI
    Shinn, P
    Conway, AB
    Vysotskaia, VS
    Dewar, K
    Conn, L
    Lenz, CA
    Kim, CJ
    Hansen, NF
    Liu, SX
    Buehler, E
    Altafi, H
    Sakano, H
    Dunn, P
    Lam, B
    Pham, PK
    Chao, Q
    Nguyen, M
    Yu, GX
    Chen, HM
    Southwick, A
    Lee, JM
    Miranda, M
    Toriumi, MJ
    Davis, RW
    [J]. NATURE, 2000, 408 (6814) : 796 - 815
  • [18] Kojima S, 1999, DNA Res, V6, P407, DOI 10.1093/dnares/6.6.407
  • [19] KOOMNEEF M, 1998, ANNU REV PLANT PHYS, V49, P345
  • [20] Coordination of plant metabolism and development by the circadian clock
    Kreps, JA
    Kay, SA
    [J]. PLANT CELL, 1997, 9 (07) : 1235 - 1244