Redox regulation of lipopolysaccharide (LPS)-induced interleukin-8 (IL-8) gene expression mediated by NF kappa B and AP-1 in human astrocytoma U373 cells

被引：78

作者：

Tanaka, C ^{[1
]}

Kamata, H ^{[1
]}

Takeshita, H ^{[1
]}

Yagisawa, H ^{[1
]}

Hirata, H ^{[1
]}

机构：

[1] HIMEJI INST TECHNOL, FAC SCI, DEPT LIFE SCI, KAMIGORI CHOU, HYOGO 67812, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 232卷 / 02期

关键词：

D O I：

10.1006/bbrc.1997.6264

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

LPS-induced expression of the IL-8 gene was markedly enhanced by H2O2 or by deprivation of the cellular antioxidant glutathione by L-buthionine-(S,R)-sulfoximine (BSO) in human astrocytoma U373 cells. In contrast, it was markedly suppressed by the reductant N-acetyl-L-cysteine (NAC) and other antioxidants. Transient expression analysis using the chloramphenicol acetyltransferase assay revealed that activation of the IL-8 promoter by LPS was stimulated by BSO and was suppressed by NAC; likewise LPS-induced activation of both NF kappa B and AP-1 was enhanced by BSO and inhibited by NAG. These results suggest that LPS-induced IL-8 gene expression is regulated by cellular redox via modulation of these transcription factors. (C) 1997 Academic Press.

引用

页码：568 / 573

页数：6

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