Experimental Definition of a Clustered Regularly Interspaced Short Palindromic Duplicon in Escherichia coli

被引：38

作者：

Goren, Moran G. ^{[1
]}

Yosef, Ido ^{[1
]}

Auster, Oren ^{[1
]}

Qimron, Udi ^{[1
]}

机构：

[1] Tel Aviv Univ, Sackler Sch Med, Dept Clin Microbiol & Immunol, IL-69978 Tel Aviv, Israel

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2012年 / 423卷 / 01期

基金：

以色列科学基金会;

关键词：

defense mechanism; bacteriophage; CRISPR adaptation; DNA template; CRISPR; PROKARYOTES; DEFENSE; REPEATS; SYSTEM;

D O I：

10.1016/j.jmb.2012.06.037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We analyzed sequences of newly inserted repeats in an Escherichia coli CRISPR (clustered regularly interspaced short palindromic repeats) array in vivo and showed that a base previously thought to belong to the repeat is actually derived from a protospacer. Based on further experimental results, we propose to use the term "duplicon" for a repeated sequence in a CRISPR array that serves as a template for a new duplicon. Our findings suggest the possibility of redrawing the borders between repeats, spacers, and protospacer adjacent motifs. (c) 2012 Elsevier Ltd. All rights reserved.

引用

页码：14 / 16

页数：3

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