HPLC enantiomeric resolution of (+)-cinchonine and (-)-cinchonidine with diode-laser polarimetric detection

The combination of UV and diode-laser polarimetric detection of chiral molecules offers significant advantages in high performance liquid chromatography. The method described here circumvents the derivatization step and the use of chiral stationary or mobile phases for the resolution of enantiomers. Data acquisition of the polarimeter was performed with AC/DC interface and software Pico ADC-100 coupled to the polarimeter detector. Quantitative methods in the range 1-150 mu g and 1-600 mu g of (+)-cinchonine and (-)-cinchonidine, respectively, were established Enantiomeric ratio determined by using UV and diode-laser polarimetric data without chiral separation is discussed.