CHIROPTICAL DETECTION DURING LIQUID-CHROMATOGRAPHY .4. APPLICATIONS TO STEREOANALYSIS AND STEREODYNAMICS

Qualitative and quantitative use of polarimetry at one wavelength for detection during liquid chromatography is briefly reviewed. Acquisition of circular dichroism spectra by stopped-flow and nonstop measurements has been further developed. Reasons are given why the angle-of rotation, i.e., the polarimeter, is preferred for monochromatic use and differential absorbance, i.e., the dichrograph, for polychromatic requirements. Both methods are demonstrated by novel applications, mainly to enantiomers which interconvert thermally via intramolecular processes.