Total chemical synthesis, folding, and assay of a small protein on a water-compatible solid support

被引：44

作者：

Johnson, Erik C. B. ^{[1
]}

Durek, Thomas ^{[1
]}

Kent, Stephen B. N. ^{[1
]}

机构：

[1] Univ Chicago, Dept Biochem, Inst Biophys Dynam, Chicago, IL 60637 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 20期

关键词：

enzymes; native chemical ligation; protein synthesis; proteins; solid-phase synthesis;

D O I：

10.1002/anie.200600381

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) All wrapped up: A protein was assembled inside a beaded polymer, then folded with concomitant formation of disulfide bonds, and assayed for biochemical activity while still bound to the water-compatible polymer. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：3283 / 3287

页数：5

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