Immunoassay detection using functionalized gold nanoparticle probes coupled with resonance Rayleigh scattering

被引：28

作者：

Cai, Huai-Hong ^{[1
]}

Yang, Pei-Hui ^{[1
]}

Feng, Jing ^{[1
]}

Cai, Jiye ^{[1
]}

机构：

[1] Jinan Univ, Dept Chem, Guangzhou 510632, Guangdong, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2009年 / 135卷 / 02期

基金：

美国国家科学基金会;

关键词：

Immunoassay; Nanoprobe; Transferrin; Resonance Rayleigh scattering; CAPACITIVE IMMUNOSENSOR; TRANSFERRIN; CANCER; HYPERTENSION; ABSORPTION; MONOLAYER; BIOMARKER; PROTEINS; NANORODS; PLASMA;

D O I：

10.1016/j.snb.2008.09.037

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Functionalized gold nanoparticle probes coupling with resonance Rayleigh scattering (RRS) has been developed for the picomolar detection of transferrin. This assay relies on the specific immune recognition of nanoprobe that is modified with anti-transferrin antibody on gold nanoparticle surface through cysteamine bioconjugation. Transmission electron microscopy (TEM), laser light scattering and UV-vis absorption spectroscopy were employed to construct and characterize the nanostructures and spectroscopic characteristics of nanoprobes. Furthermore, the experiment conditions, such as nanoprobe concentration, reaction temperature, incubation time, pH value, salt concentration and coexisting substances, are optimized. The combination of signal amplification of nanoprobes with high sensitivity of RRS technique allow the detection limit of transferrin to reach 85 pM, and the linear detection range from 85 pM to 3.4 nM. This method has been Successfully applied to detect transferrin of human serum samples with good reproducibility, and it has the potential of being widely used in clinical diagnosis. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：603 / 609

页数：7

共 32 条

[1] Fluoroimmunoassay for antigen based on fluorescence quenching signal of gold nanoparticles [J].

Ao, LM ;

Gao, F ;

Pan, BF ;

He, R ;

Cui, DX .

ANALYTICAL CHEMISTRY, 2006, 78 (04) :1104-1106

[2] Nanoprobe-based affinity mass spectrometry for selected protein profiling in human plasma [J].

Chou, PH ;

Chen, SH ;

Liao, HK ;

Lin, PC ;

Her, GR ;

Lai, ACY ;

Chen, JH ;

Lin, CC ;

Chen, YJ .

ANALYTICAL CHEMISTRY, 2005, 77 (18) :5990-5997

[3]

Dong Q, 2001, ELECTROPHORESIS, V22, P128, DOI 10.1002/1522-2683(200101)22:1<128::AID-ELPS128>3.0.CO

[4]

2-L

[5] Surface plasmon resonance scattering and absorption of anti-EGFR antibody conjugated gold nanoparticles in cancer diagnostics: Applications in oral cancer [J].

El-Sayed, IH ;

Huang, XH ;

El-Sayed, MA .

NANO LETTERS, 2005, 5 (05) :829-834

[6] Carbohydrate-deficient transferrin: Validity of a new alcohol biomarker in a sample of patients with diabetes and hypertension [J].

Fleming, M ;

Mundt, M .

JOURNAL OF THE AMERICAN BOARD OF FAMILY PRACTICE, 2004, 17 (04) :247-255

[7] Biosensing based on light absorption of nanoscaled gold and silver particles [J].

Frederix, F ;

Friedt, JM ;

Choi, KH ;

Laureyn, W ;

Campitelli, A ;

Mondelaers, D ;

Maes, G ;

Borghs, G .

ANALYTICAL CHEMISTRY, 2003, 75 (24) :6894-6900

[8] Selective surface activation of a functional monolayer for the fabrication of nanometer scale thiol patterns and directed self-assembly of gold nanoparticles [J].

Fresco, ZM ;

Fréchet, JMJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (23) :8302-8303

[9] Detection by ELISA of low transferrin levels in bronchoalveolar secretions of preterm infants [J].

Gerber, CE ;

Bruchelt, G ;

Götze-Speer, B ;

Speer, CP .

JOURNAL OF IMMUNOLOGICAL METHODS, 2000, 233 (1-2) :41-45

[10] Sensitive and simple determination of protein by resonance Rayleigh scattering with 4-azochromotropic acid phenylfluorone [J].

Guo, ZX ;

Shen, HX .

ANALYTICA CHIMICA ACTA, 2000, 408 (1-2) :177-182

← 1 2 3 4 →