Two different clades of copia-like retrotransposons in the red alga, Porphyra yezoensis

被引:8
作者
Peddigari, Suresh [1 ]
Zhang, Wenbo [1 ]
Takechi, Katsuaki [1 ]
Takano, Hiroyoshi [1 ]
Takio, Susumu [2 ]
机构
[1] Kumamoto Univ, Grad Sch Sci & Technol, Kumamoto 8608555, Japan
[2] Kumamoto Univ, Ctr Marine Environm Studies, Kumamoto 8608555, Japan
关键词
Transposable element; Reverse transcriptase; Integrase; Rhodophyta;
D O I
10.1016/j.gene.2008.07.021
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A copia-like retrotransposon referred to as PyRE1G1 was isolated from the genome of the red alga Porphyra yezoensis. PyRE1G1 is 4807 bp in length, with 204 bp long terminal repeats (LTRs) at both ends. PyRE1G1 has an open reading frame of 1401 residues encoding gag, protease, integrase, reverse transcriptase (RT), and RNase H. From the order of gene arrangement of proteins, PyRE1G1 appears to be a copia-like retrotransposon. Genomic Southern blot analysis suggests that PyRE1G1 consists of a small gene family. From the phylogenetic tree of RT sequences, PyRE1G1 is grouped in the clade of usual copia elements and distinct from the previously isolated red algal copia-like gene PyRE10G in that the latter is closely related to a new clade of aquatic animal-specific copia-like retrotransposons. (c) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:153 / 158
页数:6
相关论文
共 30 条
[21]   A Copia-like retrotransposon gene encoding Gypsy-like integrase in a red alga, Porphyra yezoensis [J].
Peddigari, Suresh ;
Zhang, Wenbo ;
Sakai, Mika ;
Takechi, Katsuaki ;
Takano, Hiroyoshi ;
Takio, Susumu .
JOURNAL OF MOLECULAR EVOLUTION, 2008, 66 (01) :72-79
[22]   REM1, a new type of long terminal repeat retrotransposon in Chlamydomonas reinhardtii [J].
Pérez-Alegre, M ;
Dubus, A ;
Fernández, E .
MOLECULAR AND CELLULAR BIOLOGY, 2005, 25 (23) :10628-10638
[23]   Genes of the Pseudoviridae (Ty1/copia retrotransposons) [J].
Peterson-Burch, BD ;
Voytas, DF .
MOLECULAR BIOLOGY AND EVOLUTION, 2002, 19 (11) :1832-1845
[24]  
PURASLUTZKE RA, 1998, J VIROL, V72, P4841
[25]   Light-dependent expression in liverwort cells of chlL/N and chlB identified as chloroplast genes involved in chlorophyll synthesis in the dark [J].
Suzuki, T ;
Takio, S ;
Satoh, T .
JOURNAL OF PLANT PHYSIOLOGY, 1998, 152 (01) :31-37
[26]   Insertional mutagenesis:: a Swiss army knife for functional genomics of Medicago truncatula [J].
Tadege, M ;
Ratet, P ;
Mysore, KS .
TRENDS IN PLANT SCIENCE, 2005, 10 (05) :229-235
[27]   GalEa retrotransposons from galatheid squat lobsters (Decapoda, Anomura) define a new clade of Ty1/copia-like elements restricted to aquatic species [J].
Terrat, Yves ;
Bonnivard, Eric ;
Higuet, Dominique .
MOLECULAR GENETICS AND GENOMICS, 2008, 279 (01) :63-73
[28]   CLUSTAL-W - IMPROVING THE SENSITIVITY OF PROGRESSIVE MULTIPLE SEQUENCE ALIGNMENT THROUGH SEQUENCE WEIGHTING, POSITION-SPECIFIC GAP PENALTIES AND WEIGHT MATRIX CHOICE [J].
THOMPSON, JD ;
HIGGINS, DG ;
GIBSON, TJ .
NUCLEIC ACIDS RESEARCH, 1994, 22 (22) :4673-4680
[29]   ORIGIN AND EVOLUTION OF RETROELEMENTS BASED UPON THEIR REVERSE-TRANSCRIPTASE SEQUENCES [J].
XIONG, Y ;
EICKBUSH, TH .
EMBO JOURNAL, 1990, 9 (10) :3353-3362
[30]   Reverse transcriptase-like sequences related to retrotransposon in a red alga, Porphyra yezoensis [J].
Zhang, Wenbo ;
Sakai, Mika ;
Lin, Xiaofei ;
Takechi, Katsuaki ;
Takano, Hiroyoshi ;
Takio, Susumu .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2006, 70 (08) :1999-2003