The Pseudomonas aeruginosa lipid A deacylase:: Selection for expression and loss within the cystic fibrosis airway

被引:104
作者
Ernst, RK
Adams, KN
Moskowitz, SM
Kraig, GM
Kawasaki, K
Stead, CM
Trent, MS
Miller, SI
机构
[1] Univ Washington, Dept Med, Seattle, WA 98195 USA
[2] Univ Washington, Dept Pediat, Seattle, WA 98195 USA
[3] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA
[4] Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA
[5] Natl Inst Infect Dis, Dept Biochem & Cell Biol, Tokyo 1628640, Japan
[6] James H Quillen Coll Med, Dept Microbiol, Johnson City, TN 37614 USA
关键词
D O I
10.1128/JB.188.1.191-201.2006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Lipopolysaccharide (LPS) is the major surface component of gram-negative bacteria, and a component of LPS, lipid A, is recognized by the innate immune system through the Toll-like receptor 4/MD-2 complex. Pseudomonas aeruginosa, an environmental gram-negative bacterium that opportunistically infects the respiratory tracts of patients with cystic fibrosis (CF), can synthesize various structures of lipid A. Lipid A from P. aeruginosa strains isolated from infants with CF has a specific structure that includes the removal of the 3 position 3-OH C10 fatty acid. Here we demonstrate increased expression of the P. aeruginosa lipid A 3-O-deacylase (PagL) in isolates from CF infants compared to that in environmental isolates. PagL activity was increased in environmental isolates by growth in medium limited for magnesium and decreased by growth at low temperature in laboratory-adapted strains of P. aeruginosa. P. aeruginosa PagL was shown to be an outer membrane protein by isopycnic density gradient centrifugation. Heterologous expression of P. aeruginosa pagL in Salmonella enterica serovar Typhimurium and Escherichia coli resulted in removal of the 3-OH C14 fatty acid from lipid A, indicating that P. aeruginosa PagL recognizes either 3-OH C10 or 3-OH C14. Finally, deacylated lipid A species were not observed in some clinical P. aeruginosa isolates from patients with severe pulmonary disease, suggesting that loss of PagL function can occur during long-term adaptation to the CF airway.
引用
收藏
页码:191 / 201
页数:11
相关论文
共 46 条
[41]   Bacterial iron sources: From siderophores to hemophores [J].
Wandersman, C ;
Delepelaire, P .
ANNUAL REVIEW OF MICROBIOLOGY, 2004, 58 :611-647
[42]   Molecular insights into the assembly and diversity of the outer core oligosaccharide in lipopolysaccharides from Escherichia coli and Salmonella [J].
Whitfield, C ;
Kaniuk, N ;
Frirdich, E .
JOURNAL OF ENDOTOXIN RESEARCH, 2003, 9 (04) :244-249
[43]   INVESTIGATION OF THE EARLY STEPS OF MOLYBDOPTERIN BIOSYNTHESIS IN ESCHERICHIA-COLI THROUGH THE USE OF IN-VIVO LABELING STUDIES [J].
WUEBBENS, MM ;
RAJAGOPALAN, KV .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (03) :1082-1087
[44]   Rapid isolation method for lipopolysaccharide and lipid A from Gram-negative bacteria [J].
Yi, EC ;
Hackett, M .
ANALYST, 2000, 125 (04) :651-656
[45]  
Yu HW, 2002, FRONT BIOSCI-LANDMRK, V7, pD442
[46]   Function of Escherichia coli MsbA, an essential ABC family transporter, in lipid A and phospholipid biosynthesis [J].
Zhou, ZM ;
White, KA ;
Polissi, A ;
Georgopoulos, C ;
Raetz, CRH .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (20) :12466-12475