Real-time observation of single-molecule fluorescence in microdroplet streams

被引：38

作者：

Barnes, MD

Lermer, N

Kung, CK

Whitten, WB

Ramsey, JM

Hill, SC

机构：

[1] Chem. and Analyt. Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6142

[2] Army Research Laboratory, Adelphi, MD 20783-1155

来源：

OPTICS LETTERS | 1997年 / 22卷 / 16期

关键词：

D O I：

10.1364/OL.22.001265

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We report real-time observation of fluorescence bursts from individual Rhodamine 6G molecules in streams of microdroplets (peak signal-to-noise ratios, approximate to 30) whose trajectories are constrained with a linear electric quadrupole. This approach offers a reasonable dynamic range in droplet size (3-12-mu m diameter) with <1% shot-to-shot size fluctuations and sensitivity comparable with that of droplet levitation techniques with at least 10(3) higher analysis rates. Applications to the study of single-molecule microcavity effects and stimulated emission are discussed.

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页码：1265 / 1267

页数：3

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