Regulation of LIM homeodomain activity in vivo:: A tetramer of dLDB and apterous confers activity and capacity for regulation by dLMO

被引:103
作者
Milán, M [1 ]
Cohen, SM [1 ]
机构
[1] European Mol Biol Lab, D-69117 Heidelberg, Germany
关键词
D O I
10.1016/S1097-2765(00)80374-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Dorsal-ventral axis formation in the Drosophila wing depends on the activity of the LIM homeodomain transcription factor Apterous and its cofactor, dLDB/Chip. We present evidence that Apterous activity depends on the formation of a LIM homeodomain dimer bridged by a dimer of cofactor. We show that Apterous activity levels are regulated in vivo by dLMO, an antagonist of homodimer formation. Making use of a constitutively active form of Apterous and dominant-negative forms of Apterous and dLDB/Chip, we show that the normal function of dLMO is to downregulate Apterous activity and that the dLMO mutant phenotype is due to excess Apterous activity. These findings may point to a general mechanism for regulation of LIM homeodomain protein activity.
引用
收藏
页码:267 / 273
页数:7
相关论文
共 32 条
[1]   Interactions of the LIM-domain-binding factor Ldb1 with LIM homeodomain proteins [J].
Agulnick, AD ;
Taira, M ;
Breen, JJ ;
Tanaka, T ;
Dawid, IB ;
Westphal, H .
NATURE, 1996, 384 (6606) :270-272
[2]  
BLAIR SS, 1994, DEVELOPMENT, V120, P1805
[3]   Organizing spatial pattern in limb development [J].
Brook, WJ ;
DiazBenjumea, FJ ;
Cohen, SM .
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1996, 12 :161-180
[4]   Wingless repression of Drosophila frizzled 2 expression shapes the wingless morphogen gradient in the wing [J].
Cadigan, KM ;
Fish, MP ;
Rulifson, EJ ;
Nusse, R .
CELL, 1998, 93 (05) :767-777
[5]   Visualization of gene expression in living adult Drosophila [J].
Calleja, M ;
Moreno, E ;
Pelaz, S ;
Morata, G .
SCIENCE, 1996, 274 (5285) :252-255
[6]   APTEROUS, A GENE REQUIRED FOR IMAGINAL DISK DEVELOPMENT IN DROSOPHILA ENCODES A MEMBER OF THE LIM FAMILY OF DEVELOPMENTAL REGULATORY PROTEINS [J].
COHEN, B ;
MCGUFFIN, ME ;
PFEIFLE, C ;
SEGAL, D ;
COHEN, SM .
GENES & DEVELOPMENT, 1992, 6 (05) :715-729
[7]  
DAWID IB, 1995, CR ACAD SCI III-VIE, V318, P295
[8]  
deCelis JF, 1996, DEVELOPMENT, V122, P2719
[9]  
deCelis JF, 1997, DEVELOPMENT, V124, P3241
[10]  
DiazBenjumea FJ, 1995, DEVELOPMENT, V121, P4215