Orf5/SolR:: a transcriptional repressor of the sol operon of Clostridium acetobutylicum?

被引：11

作者：

Thormann, K ^{[1
]}

Dürre, P ^{[1
]}

机构：

[1] Univ Ulm, D-89069 Ulm, Germany

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 2001年 / 27卷 / 05期

关键词：

Clostridium acetobutylicum; glycosylation; Orf5/SolR; repressor; solventogenesis;

D O I：

10.1038/sj.jim.7000144

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The gene of Orf5 (SolR) of Clostridium acetobutylicum DSM 792 was subcloned and overexpressed in Escherichia coli. The protein was purified with Ni-NTA agarose and used for DNA binding assays. No DNA binding of Orf5 to regions upstream of the sol operon from C. acetobutylicum was observed. Overexpression of Orf5 in C. acetobutylicum led to a change in the organism's pattern of glycosylated exoproteins. The Orf5 protein was localized in the cell membrane fraction and to a small extent in the supernatant medium. Based on these results Orf5 (SolR) appears not to act as a transcriptional repressor in C. acetobutylicum, but instead may be an enzyme involved in glycosylation or deglycosylation.

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页码：307 / 313

页数：7

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