Nitric oxide release during evoked neuronal activity in cerebellum slices: Detection with platinized carbon-fiber microelectrodes

被引:57
作者
Amatore, C
Arbault, S
Bouret, Y
Cauli, B
Guille, M
Rancillac, A
Rossier, J
机构
[1] UPMV, ENS, UMR CNRS 8640, Dept Chim, F-75231 Paris 05, France
[2] ESPCI, Lab Neurobiol & Divers Cellulaire, UMR CNRS 7637, F-75005 Paris, France
关键词
D O I
10.1002/cphc.200500202
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Nitric oxide is on important biological messenger that particularly induces the relaxation of smooth muscle cells surrounding vessels, and, hence, controls the flow of blood. This mechanism is essential for brain function, and its fine control, termed functional hyperemia, is supposed to be realized by certain neurons that may release bursts of NO.. The aim of the present study is to examine the advantages of platinized carbon-fiber microelectrodes (5-7 mu m tip diameter) for the direct and in situ electrochemical detection of NO. released by neurons into ex vivo cerebellum slices. After establishing the different analytical properties of the platinized carbon-fiber microelectrodes in vitro on NO. solutions at 50 nm to 1 mm concentration, they were characterized using DEA-NONOote solutions that chemically decompose into No-. and therefore mimic the measurement of transient variations of NO. concentration in biological samples. This validated the present approach, so that direct, in situ ex vivo measurements of nitric oxide released by neurons in a rat cerebellar slice are presented and discussed.
引用
收藏
页码:181 / 187
页数:7
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