Effects of Proteins from Culture Medium on Surface Property of Silanes- Functionalized Magnetic Nanoparticles

被引：62

作者：

Chen, Z. P. ^{[1
,2
]}

Xu, R. Z. ^{[1
]}

Zhang, Y. ^{[1
]}

Gu, N. ^{[1
]}

机构：

[1] Southeast Univ, Sch Biol Sci & Med Engn, Jiangsu Key Lab Biomat & Devices, State Key Lab Bioelect, Nanjing 210096, Peoples R China

[2] Southeast Univ, Sch Chem & Chem Engn, Nanjing 210096, Peoples R China

来源：

NANOSCALE RESEARCH LETTERS | 2009年 / 4卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Magnetic nanoparticles; Culture medium; Protein; Surface property; Cellular uptake; WALLED CARBON NANOTUBES; INTRACELLULAR UPTAKE; SUPERPARAMAGNETIC NANOPARTICLES; OXIDE NANOPARTICLES; LIGAND-EXCHANGE; CONTRAST AGENTS; CELLULAR UPTAKE; CANCER-CELLS; NANOCRYSTALS; ACID;

D O I：

10.1007/s11671-008-9226-1

中图分类号：

TB3 [工程材料学];

学科分类号：

082905 [生物质能源与材料];

摘要：

Monodisperse magnetic nanoparticles (MNPs) were synthesized by thermal decomposition of iron-oleate and functionalized with silanes bearing various functional groups such as amino group (NH2), short-chain poly(ethylene glycol) (PEG), and carboxylic group (COOH). Then, silanes-functionalized magnetic nanoparticles (silanes-MNPs) were incubated in cell culture medium plus fetal calf serum to investigate the effects of proteins from culture medium on surface property of MNPs. Zeta potential measurements showed that although surface charges of silanes-MNPs were different, they exhibited negative charges at neutral pH and approximate isoelectric points after they were incubated in cell culture medium. The reason was that silanes-MNPs could easily adsorb proteins from culture medium via non-covalent binding, resulting in the formation of protein-silanes-MNPs conjugates. Moreover, silanes-MNPs with various functional groups had different adsorption capacity to proteins, as confirmed by Coomassie blue fast staining method. The in vitro cell experiments showed that protein-silanes-MNPs had higher cellular uptake by cancer cells than silanes-MNPs.

引用

页码：204 / 209

页数：6

共 28 条

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Probing the interaction of single walled carbon nanotubes within cell culture medium as a precursor to toxicity testing [J].