Genome-wide expression analysis of yeast response during exposure to 4°C

被引:67
作者
Murata, Y
Homma, T
Kitagawa, E
Momose, Y
Sato, MS
Odani, M
Shimizu, H
Hasegawa-Mizusawa, M
Matsumoto, R
Mizukami, S
Fujita, K
Parveen, M
Komatsu, Y
Iwahashi, H
机构
[1] Natl Inst Adv Ind Sci & Technol, Int Patent Organism Depositary, Tsukuba, Ibaraki 3058566, Japan
[2] Natl Inst Adv Ind Sci & Technol, Human Stress Signal Res Ctr, Tsukuba, Ibaraki 3058566, Japan
[3] Daicel Chem Ind Ltd, JBA, Tsukuba, Ibaraki, Japan
[4] Natl Inst Adv Ind Sci &Technol, Res Inst Biol Resources & Funct, Tsukuba, Ibaraki 3058566, Japan
[5] Natl Inst Technol & Evaluat, Chiba 2920818, Japan
[6] Kyoto Univ, Inst Frontier Med Sci, Dept Mol & Cellular Biol, Sakyo Ku, Kyoto 6068507, Japan
关键词
yeast; DNA microarray; hierarchical cluster; gene expression profiling; cold shock protein; trehalose; glycogen; membrane maintenance;
D O I
10.1007/s00792-005-0480-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Adaptation to temperature fluctuation is essential for the survival of all living organisms. Although extensive research has been done on heat and cold shock responses, there have been no reports on global responses to cold shock below 10 degrees C or near-freezing. We examined the genome-wide expression in Saccharomyces cerevisiae, following exposure to 4 degrees C. Hierarchical cluster analysis showed that the gene expression profile following 4 degrees C exposure from 6 to 48 h was different from that at continuous 4 degrees C culture. Under 4 degrees C exposure, the genes involved in trehalose and glycogen synthesis were induced, suggesting that biosynthesis and accumulation of those reserve carbohydrates might be necessary for cold tolerance and energy preservation. The observed increased expression of phospholipids, mannoproteins, and cold shock proteins (e.g., TIP1) is consistent with membrane maintenance and increased permeability of the cell wall at 4 degrees C. The induction of heat shock proteins and glutathione at 4 degrees C may be required for revitalization of enzyme activity, and for detoxification of active oxygen species, respectively. The genes with these functions may provide the ability of cold tolerance and adaptation to yeast cells.
引用
收藏
页码:117 / 128
页数:12
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