A high-throughput approach to identify genomic variants of bacterial metabolite producers at the single-cell level

被引:199
作者
Binder, Stephan [1 ]
Schendzielorz, Georg [1 ]
Staebler, Norma [1 ]
Krumbach, Karin [1 ]
Hoffmann, Kristina [1 ]
Bott, Michael [1 ]
Eggeling, Lothar [1 ]
机构
[1] Forschungszentrum Julich, Inst Bio & Geowissensch, IBG Biotechnol 1, D-52425 Julich, Germany
来源
GENOME BIOLOGY | 2012年 / 13卷 / 05期
关键词
CORYNEBACTERIUM-GLUTAMICUM; BIOSENSORS; BIOSYNTHESIS; INFORMATION; SPECIFICITY; TRANSPORT; PRECURSOR; ARGININE;
D O I
10.1186/gb-2012-13-5-r40
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We present a novel method for visualizing intracellular metabolite concentrations within single cells of Escherichia coli and Corynebacterium glutamicum that expedites the screening process of producers. It is based on transcription factors and we used it to isolate new L-lysine producing mutants of C. glutamicum from a large library of mutagenized cells using fluorescence-activated cell sorting (FACS). This high-throughput method fills the gap between existing high-throughput methods for mutant generation and genome analysis. The technology has diverse applications in the analysis of producer populations and screening of mutant libraries that carry mutations in plasmids or genomes.
引用
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页数:12
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