Novel natural genetic variation controlling the competence to form adventitious roots and shoots from the tomato wild relative Solanum pennellii

被引:7
作者
Arikita, Fernanda Namie [1 ]
Azevedo, Mariana Silva [1 ,2 ]
Scotton, Danielle Camargo [1 ,2 ]
Pinto, Maisa de Siqueira [1 ]
Figueira, Antonio [2 ]
Pereira Peres, Lazaro Eustaquio [1 ]
机构
[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz ESALQ, Dept Biol Sci LCB, Lab Hormonal Control Plant Dev, BR-13418900 Piracicaba, SP, Brazil
[2] Univ Sao Paulo, Ctr Energia Nucl Agr CENA, BR-13400970 Piracicaba, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
Introgression lines; Micro-Tom; Regeneration; Rg1; S; lycopersicum; CULTURED LEAF EXPLANTS; IN-VITRO; PLANT-REGENERATION; LYCOPERSICON-ESCULENTUM; MICRO-TOM; MERISTEM GENE; MODEL SYSTEM; QTL ANALYSIS; CAPACITY; CALLUS;
D O I
10.1016/j.plantsci.2012.11.005
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Tomato (Solanum lycopersicum L.) is an attractive model to study the genetic basis of adventitious organ formation capacity, since there is considerable natural genetic variation among wild relatives. Using a set of 46 introgression lines (ILs), each containing a small chromosomal segment of Solanum pennellii LA716 introgressed and mapped into the tomato cultivar M82, we characterized a high shoot-regeneration capacity for ILs 3-2, 6-1, 7-1, 7-2, 8-2, 8-3, 9-1, 9-2, 10-2 and 10-3, when cotyledon explants were cultivated on medium containing 5.0 mu M BAP. F1 seedlings from the crosses 'Micro-Tom x ILs' and 'ILs x ILs' demonstrated that the shoot regeneration capacity of most ILs was dominant and that the regeneration ability of IL8-3 enhanced that of the other ILs in an additive manner. The ILs 3-2, 7-1, 8-3, and 10-2 also exhibited enhanced root formation on MS medium containing 0.4 mu M NAA, indicating that these chromosomal segments may contain genes controlling the competence to assume distinct cell fates, rather than the induction of a specific organ. We also performed the introgression of the genes controlling competence into the model system 'Micro-Tom'. The further isolation of such genes will improve our understanding of the molecular basis of organogenic capacity. (c) 2012 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:121 / 130
页数:10
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