Detection of DNA in agarose gels using berberine and Mordant Yellow 3R

被引：11

作者：

Jung, DW

Yoo, GS

Choi, JK ^{[1
]}

机构：

[1] Chonnam Natl Univ, Coll Pharm, Kwangju 500757, South Korea

[2] Chonnam Natl Univ, Res Inst Drug Dev, Kwangju 500757, South Korea

来源：

ANALYTICAL BIOCHEMISTRY | 1999年 / 272卷 / 02期

关键词：

berberine; Mordant Yellow 3R; DNA; agarose gels; counterion dye;

D O I：

10.1006/abio.1999.4198

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A nontoxic and simple staining method for the detection of DNA in agarose gels is described. After eletrophoretic separation, the gels were stained with 5 mu g/ml of berberine (BB) prepared in distilled water and then the gels were soaked in 20 mu g/ml of aqueous Mordant Yellow 3R (MY3R) solution. Employment of MY3R as a counterion dye efficiently quenched unwanted background fluorescence of BE. This method can detect as little as 10 ng of plasma membrane H+-ATPase cDNA obtained from Arabidopsis thaliana L. (AHA1, 3.2 kb) under a long wavelength of UV irradiation (366 nm) within 1 h. (C) 1999 Academic Press.

引用

收藏

页码：254 / 256

页数：3

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