Metabolic characterization of the R6/2 transgenic mouse model of Huntington's disease by high-resolution MAS 1H NMR spectroscopy

被引：101

作者：

Tsang, TA

Woodman, B

McLoughlin, GA

Griffin, JL

Tabrizi, SJ

Bates, GP

Holmes, E

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Fac Med, Div Biomed Sci, London SW7 2AZ, England

[2] Kings Coll London, GKT Sch Med, London SE1 9RT, England

[3] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England

[4] UCL, Inst Neurol, Dept Neurodegenerat Dis, London WC1N 3BG, England

来源：

JOURNAL OF PROTEOME RESEARCH | 2006年 / 5卷 / 03期

基金：

英国惠康基金;

关键词：

Huntington's disease; metabolic profiling; NMR spectroscopy; pattern recognition; R6/2; mice;

D O I：

10.1021/pr050244o

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The metabolic consequences of Huntington's disease in the R6/2 mouse model were investigated using NMR spectroscopy and pattern recognition to characterize selected brain regions, muscle, blood, and urine. Global increases in relative brain concentrations of osmolytes, creatine, glutamine, and lactate, and decreases in acetate and N-acetylaspartate were found together with striatal-specific lower concentrations of GABA and choline. Clear differentiation of R6/2 and wild-type mice was also obtained for urine and blood metabolite profiles that may have applicability for monitoring HD in human populations.

引用

页码：483 / 492

页数：10

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