Characterization of a cDNA and gene encoding the mouse Theta class glutathione transferase mGSTT2 and its localization to chromosome 10B5-C1

被引：18

作者：

Whittington, AT ^{[1
]}

Webb, GC ^{[1
]}

Baker, RT ^{[1
]}

Board, PG ^{[1
]}

机构：

[1] QUEEN ELIZABETH HOSP, DEPT OBSTET & GYNAECOL, WOODVILLE, SA 5011, AUSTRALIA

来源：

GENOMICS | 1996年 / 33卷 / 01期

关键词：

D O I：

10.1006/geno.1996.0165

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, we have isolated and characterized a gene and cDNA encoding a mouse Theta class GST. The gene, mGSTT2, spans approximately 3.1 kb and is composed of five exons interrupted by four introns. The gene was localized to Chromosome 10B5-C1 by in situ hybridization. Southern blot analysis of mouse genomic DNA suggests that there is only one copy of mGSTT2 in the mouse genome. The cDNA derived from mGSTT2 was isolated from a mouse liver cDNA library and has an open reading frame of 732 bp encoding a peptide of 244 amino acids with a calculated molecular weight of 26,676 Da. The encoded protein shares amino acid sequence identities of 92, 77, 51, and 55% with rat subunit Yrs, human subunit GSTT2, rat subunit 5, and human subunit GSTT1, respectively. (C) 1996 Academic Press, Inc.

引用

页码：105 / 111

页数：7

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