High ethanol tolerance of new isolates of Clostridium thermocellum strains SS21 and SS22

被引：23

作者：

Rani, KS ^{[1
]}

Seenayya, G ^{[1
]}

机构：

[1] Osmania Univ, Dept Microbiol, Hyderabad 7, Andhra Pradesh, India

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1999年 / 15卷 / 02期

关键词：

anaerobic bacteria; biomass to ethanol; ethanol tolerance; growth temperature; solvent tolerance; thermophilic bacteria;

D O I：

10.1023/A:1008863410460

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Clostridium thermocellum strains SS21 and SS22, producing high yields of ethanol, were tolerant to 4.0 and 5.0% (v/v) ethanol, respectively. This is the highest ethanol tolerance so far reported by wild type strains of C. thermocellum. In the presence of added ethanol, both the strains had extended period of growth arrest. On addition of ethanol at different culture ages increase in ethanol tolerance upto 7.0 and 8.0% (v/v) by strains SS21 and SS22, respectively was observed. The optimum growth temperature for strain SS21 decreased as the concentration of ethanol in the medium increased and remained constant for strain SS22. Both the strains were tolerant to various solvents and acetic acid indicating that high ethanol tolerance of the strains is due to the general solvent tolerance of the organisms.

引用

页码：173 / 178

页数：6

共 25 条

[21]

RANI KS, 1997, BIOTECHNOL LETT, V8, P819

[22] ENHANCED CELLULOSE FERMENTATION BY AN ASPOROGENOUS AND ETHANOL-TOLERANT MUTANT OF CLOSTRIDIUM-THERMOCELLUM [J].

TAILLIEZ, P ;

GIRARD, H ;

MILLET, J ;

BEGUIN, P .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (01) :207-211

[23] CELLULOSE FERMENTATION BY AN ASPOROGENOUS MUTANT AND AN ETHANOL-TOLERANT MUTANT OF CLOSTRIDIUM-THERMOCELLUM [J].

TAILLIEZ, P ;

GIRARD, H ;

LONGIN, R ;

BEGUIN, P ;

MILLET, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (01) :203-206

[24] ETHANOL FROM CELLULOSIC BIOMASS [J].

WANG, DIC ;

AVGERINOS, GC ;

BIOCIC, I ;

WANG, SD ;

FANG, HY .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 1983, 300 (1100) :323-333

[25] FERMENTATION OF CELLULOSE AND CELLOBIOSE BY CLOSTRIDIUM-THERMOCELLUM IN ABSENCE AND PRESENCE OF METHANOBACTERIUM-THERMOAUTOTROPHICUM [J].

WEIMER, PJ ;

ZEIKUS, JG .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1977, 33 (02) :289-297

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