The dynamics of gene expression in vertebrate embryogenesis at single-cell resolution

被引：385

作者：

Briggs, James A. ^{[1
]}

Weinreb, Caleb ^{[1
]}

Wagner, Daniel E. ^{[1
]}

Megason, Sean ^{[1
]}

Peshkin, Leonid ^{[1
]}

Kirschner, Marc W. ^{[1
]}

Klein, Allon M. ^{[1
]}

机构：

[1] Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA

来源：

SCIENCE | 2018年 / 360卷 / 6392期

关键词：

MAJOR DEVELOPMENTAL TRANSITION; EARLY XENOPUS-EMBRYOS; STEM-CELLS; LINEAGE COMMITMENT; DIFFERENTIATION; ORIGIN; LAEVIS; FATE; PLURIPOTENCY; ENDODERM;

D O I：

10.1126/science.aar5780

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Time series of single-cell transcriptome measurements can reveal dynamic features of cell differentiation pathways. From measurements of whole frog embryos spanning zygotic genome activation through early organogenesis, we derived a detailed catalog of cell states in vertebrate development and a map of differentiation across all lineages over time. The inferred map recapitulates most if not all developmental relationships and associates new regulators and marker genes with each cell state. We find that many embryonic cell states appear earlier than previously appreciated. We also assess conflicting models of neural crest development. Incorporating a matched time series of zebrafish development from a companion paper, we reveal conserved and divergent features of vertebrate early developmental gene expression programs. © The Authors, some rights reserved.

引用

页码：980 / +

页数：10

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