Unconventional secretion of tubby and tubby-like protein 1

被引：22

作者：

Caberoy, Nora B. ^{[1
]}

Li, Wei ^{[1
]}

机构：

[1] Univ Miami, Sch Med, Dept Ophthalmol, Bascom Palmer Eye Inst, Miami, FL 33136 USA

来源：

FEBS LETTERS | 2009年 / 583卷 / 18期

关键词：

Tubby; Tubby-like protein 1; Unconventional secretion; Extracellular trafficking; Obesity; Retinal degenerations; GENE FAMILY; RETINAL DEGENERATION; RETINITIS-PIGMENTOSA; PLASMA-MEMBRANE; MUTATION; TULP1; EXPORT; CELLS; FIBROBLAST-GROWTH-FACTOR-2; EXPRESSION;

D O I：

10.1016/j.febslet.2009.08.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tubby-like proteins (Tulps) with no signal peptide have been characterized as cytoplasmic proteins with various intracellular functions, including binding to phosphatidylinositol-4,5-bisphosphate [PI(4,5)P-2]. PI(4,5)P-2 has been implicated in unconventional secretion of. broblast growth factor-2 without a signal peptide. Here, we show that all Tulps are expressed intracellularly and extracellularly. Tubby secretion is partially dependent on its PI(4,5)P-2-binding activity with an essential secretory signal in the N-terminus. Pathogenic mutation in Tubby mice has no impact on tubby extracellular trafficking. Moreover, unconventional secretion of tubby and Tulp1 is independent of endoplasmic reticulum-Golgi pathway. These data implicate that Tulps may function extracellularly as well. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：3057 / 3062

页数：6

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