Genetic characterization of Bagaza virus (BAGV) isolated in India and evidence of anti-BAGV antibodies in sera collected from encephalitis patients

被引：48

作者：

Bondre, Vijay P. ^{[1
]}

Sapkal, Gajanan N. ^{[1
]}

Yergolkar, Prasanna N. ^{[2
]}

Fulmali, Pradip V. ^{[1
]}

Sankararaman, Vasudha ^{[1
]}

Ayachit, Vijay M. ^{[1
]}

Mishra, Akhilesh C. ^{[1
]}

Gore, Milind M. ^{[1
]}

机构：

[1] Natl Inst Virol, Pune 411021, Maharashtra, India

[2] Natl Inst Virol, Bangalore Field Unit, Bangalore, Karnataka, India

来源：

JOURNAL OF GENERAL VIROLOGY | 2009年 / 90卷

关键词：

WEST-NILE-VIRUS; RIFT-VALLEY-FEVER; JAPANESE ENCEPHALITIS; FLAVIVIRUSES; EPIDEMIOLOGY; EMERGENCE;

D O I：

10.1099/vir.0.012336-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

During investigations into the outbreak of encephalitis in 1996 in the Kerala state in India, an arbovirus was isolated from a Culex tritaeniorhynchus mosquito pool. It was characterized as a Japanese encephalitis and West Nile virus cross-reactive arbovirus by complement fixation test. A plaque reduction-neutralization test was performed using hyperimmune sera raised against the plaque-purified arbovirus isolate. The sera did not show reactivity with Japanese encephalitis virus and were weakly reactive with West Nile virus. Complete open reading frame sequence analysis characterized the arbovirus as Bagaza virus (BAGV), with 94.80% nucleotide identity with African BAGV strain DakAr B209. Sera collected from the encephalitic patients during the acute phase of illness showed 15% (8/53) positivity for anti-BAGV neutralizing antibodies. This is the first report of the isolation of BAGV from India. The presence of anti-BAGV neutralizing antibodies suggests that the human population has been exposed to BAGV.

引用

页码：2644 / 2649

页数：6

共 23 条

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