Bergmann glial cells in situ express endothelin(B) receptors linked to cytoplasmic calcium signals

被引:41
作者
Tuschick, S
Kirischuk, S
Kirchhoff, F
Liefeldt, L
Paul, M
Verkhratsky, A
Kettenmann, H
机构
[1] MAX DELBRUCK CTR MOL MED,D-13122 BERLIN,GERMANY
[2] FREE UNIV BERLIN,BENJAMIN FRANKLIN HOSP,DEPT CLIN PHARMACOL,D-1000 BERLIN,GERMANY
关键词
D O I
10.1016/S0143-4160(97)90052-X
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The endothelin (ET) isoforms ET-1, ET-2 and ET-3 applied at 100 nM triggered a transient increase in [Ca2+](i) in Bergmann glial cells in cerebellar slices acutely isolated from 20-25 day-old mice. The intracellular calcium concentration ([Ca2+](i)) was monitored using Fura-2-based [Ca2+](i) microfluorimetry. The ET-triggered [Ca2+](i) transients were mimicked by ETB receptor agonist BQ-3020 and were inhibited by ETB receptor antagonist BQ-788. ET elevated [Ca2+](i) in Ca2+-free extracellular solution and the ET-triggered [Ca2+](i) elevation was blocked by 500 nM thapsigargin indicating that the [Ca2+](i) was released from InsP(3)-sensitive intracellular pools. The ET-triggered [Ca2+](i) increase in Ca2+-free solution was shorter in duration. Restoration of normal extracellular [Ca2+] briefly after the ET application induced a second [Ca2+](i) increase indicating the presence of a secondary Ca2+ influx which prolongs the Ca2+ signal. Pre-application of 100 mu M ATP or 10 mu M noradrenaline blocked the ET response suggesting the involvement of a common Ca2+ depot. The expression of ETB receptor mRNAs in Bergmann glial cells was revealed by single-cell RT-PCR. The mRNA was also found in Purkinje neurones, but no Ca2+ signalling was triggered by ET. We conclude that Bergmann glial cells are endowed with functional ETB receptors which induce the generation of intracellular [Ca2+](i) signals by activation of Ca2+ release from InsP(3)-sensitive intracellular stores followed by a secondary Ca2+ influx.
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页码:409 / 419
页数:11
相关论文
共 41 条
[1]  
ARAI H, 1993, J BIOL CHEM, V268, P3463
[2]   ENDOTHELIN LEVELS INCREASE IN RAT FOCAL AND GLOBAL-ISCHEMIA [J].
BARONE, FC ;
GLOBUS, MYT ;
PRICE, WJ ;
WHITE, RF ;
STORER, BL ;
FEUERSTEIN, GZ ;
BUSTO, R ;
OHLSTEIN, EH .
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 1994, 14 (02) :337-342
[3]   CAPACITATIVE CALCIUM-ENTRY [J].
BERRIDGE, MJ .
BIOCHEMICAL JOURNAL, 1995, 312 :1-11
[4]  
CRAWFORD MLA, 1990, N-S ARCH PHARMACOL, V341, P268
[5]  
ELSHOURBAGY NA, 1992, MOL PHARMACOL, V41, P465
[6]  
FERNANDEZDURANGO R, 1994, J NEUROCHEM, V62, P1482
[7]   PEPTIDIC ENDOTHELIN-1 RECEPTOR ANTAGONIST, BQ-123, AND NEUROPROTECTION [J].
FEUERSTEIN, G ;
GU, JL ;
OHLSTEIN, EH ;
BARONE, FC ;
YUE, TL .
PEPTIDES, 1994, 15 (03) :467-469
[8]  
GIAID A, 1991, HISTOCHEMISTRY, V95, P303
[9]   ENDOTHELIN INDUCES A SUSTAINED RISE IN INTRACELLULAR CALCIUM IN HIPPOCAMPAL ASTROCYTES [J].
GOLDMAN, RS ;
FINKBEINER, SM ;
SMITH, SJ .
NEUROSCIENCE LETTERS, 1991, 123 (01) :4-8
[10]  
GRYNKIEWICZ G, 1985, J BIOL CHEM, V260, P3440